A Rapid Point-of-Care Assay for Cysticercosis Antigen Detection in Urine Samples

Luz Toribio; Sukwan Handali; Yazmin Marin; Erika Perez; Yesenia Castillo; Javier A. Bustos; Seth E. O’Neal; Hector H. Garcia

doi:10.4269/ajtmh.22-0598

A Rapid Point-of-Care Assay for Cysticercosis Antigen Detection in Urine Samples

Luz Toribio
, Sukwan Handali
, Yazmin Marin
, Erika Perez
, Yesenia Castillo
, Javier A. Bustos
, Seth E. O’Neal
, Hector H. Garcia

Universidad Peruana Cayetano Heredia
Instituto Nacional de Ciencias Neurológicas
Centers for Disease Control and Prevention
Oregon Health and Science University
Asociación Benéfica PRISMA
Johns Hopkins Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

We report a proof-of-concept study using a dipstick assay to detect Taenia solium antigen in urine samples of 30 patients with subarachnoid neurocysticercosis and 10 healthy control subjects. Strips were read in blind by two readers. The assay detected antigen in 29 of 30 cases and was negative in all 10 control samples. Although this study was performed in samples from individuals with subarachnoid neurocysticercosis who likely had high circulating antigen levels, it provides the proof of concept for a functional urine antigen point-of-care assay that detects viable cysts. Such an assay could serve to support a clinical diagnosis of suspect neurocysticercosis or to identify patients at risk of developing severe disease in areas where medical resources are limited, providing evidence to refer these individuals for imaging and specialized care as needed.

Original language	English
Pages (from-to)	578-580
Number of pages	3
Journal	American Journal of Tropical Medicine and Hygiene
Volume	108
Issue number	3
DOIs	https://doi.org/10.4269/ajtmh.22-0598
State	Published - Mar 2023

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title = "A Rapid Point-of-Care Assay for Cysticercosis Antigen Detection in Urine Samples",

abstract = "We report a proof-of-concept study using a dipstick assay to detect Taenia solium antigen in urine samples of 30 patients with subarachnoid neurocysticercosis and 10 healthy control subjects. Strips were read in blind by two readers. The assay detected antigen in 29 of 30 cases and was negative in all 10 control samples. Although this study was performed in samples from individuals with subarachnoid neurocysticercosis who likely had high circulating antigen levels, it provides the proof of concept for a functional urine antigen point-of-care assay that detects viable cysts. Such an assay could serve to support a clinical diagnosis of suspect neurocysticercosis or to identify patients at risk of developing severe disease in areas where medical resources are limited, providing evidence to refer these individuals for imaging and specialized care as needed.",

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T1 - A Rapid Point-of-Care Assay for Cysticercosis Antigen Detection in Urine Samples

AU - Toribio, Luz

AU - Handali, Sukwan

AU - Marin, Yazmin

AU - Perez, Erika

AU - Castillo, Yesenia

AU - Bustos, Javier A.

AU - O’Neal, Seth E.

AU - Garcia, Hector H.

PY - 2023/3

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N2 - We report a proof-of-concept study using a dipstick assay to detect Taenia solium antigen in urine samples of 30 patients with subarachnoid neurocysticercosis and 10 healthy control subjects. Strips were read in blind by two readers. The assay detected antigen in 29 of 30 cases and was negative in all 10 control samples. Although this study was performed in samples from individuals with subarachnoid neurocysticercosis who likely had high circulating antigen levels, it provides the proof of concept for a functional urine antigen point-of-care assay that detects viable cysts. Such an assay could serve to support a clinical diagnosis of suspect neurocysticercosis or to identify patients at risk of developing severe disease in areas where medical resources are limited, providing evidence to refer these individuals for imaging and specialized care as needed.

AB - We report a proof-of-concept study using a dipstick assay to detect Taenia solium antigen in urine samples of 30 patients with subarachnoid neurocysticercosis and 10 healthy control subjects. Strips were read in blind by two readers. The assay detected antigen in 29 of 30 cases and was negative in all 10 control samples. Although this study was performed in samples from individuals with subarachnoid neurocysticercosis who likely had high circulating antigen levels, it provides the proof of concept for a functional urine antigen point-of-care assay that detects viable cysts. Such an assay could serve to support a clinical diagnosis of suspect neurocysticercosis or to identify patients at risk of developing severe disease in areas where medical resources are limited, providing evidence to refer these individuals for imaging and specialized care as needed.

UR - https://www.scopus.com/pages/publications/85149174944

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DO - 10.4269/ajtmh.22-0598

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AN - SCOPUS:85149174944

SN - 0002-9637

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SP - 578

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JO - American Journal of Tropical Medicine and Hygiene

JF - American Journal of Tropical Medicine and Hygiene

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ER -

A Rapid Point-of-Care Assay for Cysticercosis Antigen Detection in Urine Samples

Abstract

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