A set of 12 microsatellite loci for genetic studies of Leishmania braziliensis

V. Rougeron; E. Waleckx; M. Hide; T. De Meeûs; J. Arevalo; A. Llanos-Cuentas; A. L. Bañuls

doi:10.1111/j.1471-8286.2007.01953.x

A set of 12 microsatellite loci for genetic studies of Leishmania braziliensis

V. Rougeron
, E. Waleckx
, M. Hide
, T. De Meeûs
, J. Arevalo
, A. Llanos-Cuentas
, A. L. Bañuls

Institut de Recherche Pour le Développement, UMR177 IRD/CIR AD INTERTRYP

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Twelve microsatellite loci of Leishmania braziliensis were examined, nine of which were developed in this work. Fifty-six Leishmania braziliensis were genotyped with these microsatellite loci. The 12 loci studied were polymorphic with the number of alleles ranging from five to 19, with a mean of 9.7 ± 4.1 and the observed heterozygosity averaging 0.425 ± 0.202. The important heterozygote deficits we observed (F_IS = 0.41, P value = 0.004) appear incompatible with the heterozygote excess expected in clonal diploids. This last result could revive the clonality/sexuality debate regarding Leishmania. This work validates the potential use of these microsatellites for population genetics analysis.

Original language	English
Pages (from-to)	351-353
Number of pages	3
Journal	Molecular Ecology Resources
Volume	8
Issue number	2
DOIs	https://doi.org/10.1111/j.1471-8286.2007.01953.x
State	Published - Mar 2008

Keywords

Leishmania braziliensis
Microsatellites
Population genetics
Population structure

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10.1111/j.1471-8286.2007.01953.x

Cite this

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title = "A set of 12 microsatellite loci for genetic studies of Leishmania braziliensis",

abstract = "Twelve microsatellite loci of Leishmania braziliensis were examined, nine of which were developed in this work. Fifty-six Leishmania braziliensis were genotyped with these microsatellite loci. The 12 loci studied were polymorphic with the number of alleles ranging from five to 19, with a mean of 9.7 ± 4.1 and the observed heterozygosity averaging 0.425 ± 0.202. The important heterozygote deficits we observed (FIS = 0.41, P value = 0.004) appear incompatible with the heterozygote excess expected in clonal diploids. This last result could revive the clonality/sexuality debate regarding Leishmania. This work validates the potential use of these microsatellites for population genetics analysis.",

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doi = "10.1111/j.1471-8286.2007.01953.x",

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T1 - A set of 12 microsatellite loci for genetic studies of Leishmania braziliensis

AU - Rougeron, V.

AU - Waleckx, E.

AU - Hide, M.

AU - De Meeûs, T.

AU - Arevalo, J.

AU - Llanos-Cuentas, A.

AU - Bañuls, A. L.

PY - 2008/3

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AB - Twelve microsatellite loci of Leishmania braziliensis were examined, nine of which were developed in this work. Fifty-six Leishmania braziliensis were genotyped with these microsatellite loci. The 12 loci studied were polymorphic with the number of alleles ranging from five to 19, with a mean of 9.7 ± 4.1 and the observed heterozygosity averaging 0.425 ± 0.202. The important heterozygote deficits we observed (FIS = 0.41, P value = 0.004) appear incompatible with the heterozygote excess expected in clonal diploids. This last result could revive the clonality/sexuality debate regarding Leishmania. This work validates the potential use of these microsatellites for population genetics analysis.

KW - Leishmania braziliensis

KW - Microsatellites

KW - Population genetics

KW - Population structure

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DO - 10.1111/j.1471-8286.2007.01953.x

M3 - Artículo

AN - SCOPUS:38949149621

SN - 1755-098X

VL - 8

SP - 351

EP - 353

JO - Molecular Ecology Resources

JF - Molecular Ecology Resources

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ER -

A set of 12 microsatellite loci for genetic studies of Leishmania braziliensis

Abstract

Keywords

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