Biochemical characterization of Leishmania (Viannia) braziliensis and Leishmania (Viannia) peruviana by isoenzyme electrophoresis

M. Arana; D. A. Evans; A. Zolessi; A. Llanos Cuentas; J. Arevalo

doi:10.1016/0035-9203(90)90025-A

Biochemical characterization of Leishmania (Viannia) braziliensis and Leishmania (Viannia) peruviana by isoenzyme electrophoresis

M. Arana
, D. A. Evans
, A. Zolessi
, A. Llanos Cuentas
, J. Arevalo

Universidad Peruana Cayetano Heredia, Instituto de Medicina Tropical Alexander von Humboldt
Mental Honorio Delgado-Hideyo Noguchi
Universidad Peruana Cayetano Heredia
London School of Hygiene and Tropical Medicine

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

Leishmanial organisms isolated from 24 patients with Andean cutaneous leishmaniais (uta) and from 7 with sylvatic leishmaniasis in both cutaneous and mucosal forms were characterized on the basis of their isoenzyme profiles for 13 enzymes using both cellulose acetate (CA) and thin-layer starch gel (TLS) electrophoretic techniques. Malate dehydrogenase (MDH) after electrophoresis on CA or TLS and mannose phosphate isomerase (MPI) on TLS were the only enzymes of 13 examined which discriminated between the organisms from patients with uta (L. (V.) peruviana) and those with sylvatic leishmaniasis (L. (V.) braziliensis). Mannose phosphate isomerase gave more clear-cut and reproducible discrimination than did MDH on either TLS or CA, and it is suggested that MPI is a reliable enzyme marker that can be used in routine TLS electrophoresis to distinguish between L. (V.) peruviana and L. (V). braziliensis.

Original language	English
Pages (from-to)	526-529
Number of pages	4
Journal	Transactions of the Royal Society of Tropical Medicine and Hygiene
Volume	84
Issue number	4
DOIs	https://doi.org/10.1016/0035-9203(90)90025-A
State	Published - Oct 1990

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title = "Biochemical characterization of Leishmania (Viannia) braziliensis and Leishmania (Viannia) peruviana by isoenzyme electrophoresis",

abstract = "Leishmanial organisms isolated from 24 patients with Andean cutaneous leishmaniais (uta) and from 7 with sylvatic leishmaniasis in both cutaneous and mucosal forms were characterized on the basis of their isoenzyme profiles for 13 enzymes using both cellulose acetate (CA) and thin-layer starch gel (TLS) electrophoretic techniques. Malate dehydrogenase (MDH) after electrophoresis on CA or TLS and mannose phosphate isomerase (MPI) on TLS were the only enzymes of 13 examined which discriminated between the organisms from patients with uta (L. (V.) peruviana) and those with sylvatic leishmaniasis (L. (V.) braziliensis). Mannose phosphate isomerase gave more clear-cut and reproducible discrimination than did MDH on either TLS or CA, and it is suggested that MPI is a reliable enzyme marker that can be used in routine TLS electrophoresis to distinguish between L. (V.) peruviana and L. (V). braziliensis.",

author = "M. Arana and Evans, \{D. A.\} and A. Zolessi and Cuentas, \{A. Llanos\} and J. Arevalo",

year = "1990",

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doi = "10.1016/0035-9203(90)90025-A",

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T1 - Biochemical characterization of Leishmania (Viannia) braziliensis and Leishmania (Viannia) peruviana by isoenzyme electrophoresis

AU - Arana, M.

AU - Evans, D. A.

AU - Zolessi, A.

AU - Cuentas, A. Llanos

AU - Arevalo, J.

PY - 1990/10

Y1 - 1990/10

N2 - Leishmanial organisms isolated from 24 patients with Andean cutaneous leishmaniais (uta) and from 7 with sylvatic leishmaniasis in both cutaneous and mucosal forms were characterized on the basis of their isoenzyme profiles for 13 enzymes using both cellulose acetate (CA) and thin-layer starch gel (TLS) electrophoretic techniques. Malate dehydrogenase (MDH) after electrophoresis on CA or TLS and mannose phosphate isomerase (MPI) on TLS were the only enzymes of 13 examined which discriminated between the organisms from patients with uta (L. (V.) peruviana) and those with sylvatic leishmaniasis (L. (V.) braziliensis). Mannose phosphate isomerase gave more clear-cut and reproducible discrimination than did MDH on either TLS or CA, and it is suggested that MPI is a reliable enzyme marker that can be used in routine TLS electrophoresis to distinguish between L. (V.) peruviana and L. (V). braziliensis.

AB - Leishmanial organisms isolated from 24 patients with Andean cutaneous leishmaniais (uta) and from 7 with sylvatic leishmaniasis in both cutaneous and mucosal forms were characterized on the basis of their isoenzyme profiles for 13 enzymes using both cellulose acetate (CA) and thin-layer starch gel (TLS) electrophoretic techniques. Malate dehydrogenase (MDH) after electrophoresis on CA or TLS and mannose phosphate isomerase (MPI) on TLS were the only enzymes of 13 examined which discriminated between the organisms from patients with uta (L. (V.) peruviana) and those with sylvatic leishmaniasis (L. (V.) braziliensis). Mannose phosphate isomerase gave more clear-cut and reproducible discrimination than did MDH on either TLS or CA, and it is suggested that MPI is a reliable enzyme marker that can be used in routine TLS electrophoresis to distinguish between L. (V.) peruviana and L. (V). braziliensis.

UR - https://www.scopus.com/pages/publications/0025106251

U2 - 10.1016/0035-9203(90)90025-A

DO - 10.1016/0035-9203(90)90025-A

M3 - Artículo

C2 - 2091345

AN - SCOPUS:0025106251

SN - 0035-9203

VL - 84

SP - 526

EP - 529

JO - Transactions of the Royal Society of Tropical Medicine and Hygiene

JF - Transactions of the Royal Society of Tropical Medicine and Hygiene

IS - 4

ER -

Biochemical characterization of Leishmania (Viannia) braziliensis and Leishmania (Viannia) peruviana by isoenzyme electrophoresis

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