Citotoxicidad inducida por veneno de serpientes peruanas sobre fibroblastos de ratón.

M. Goñi; A. Vaisberg; A. Zavaleta

Citotoxicidad inducida por veneno de serpientes peruanas sobre fibroblastos de ratón.

Translated title of the contribution: Cytotoxicity induced by Peruvian snake venom on fibroblasts of mice

M. Goñi
, A. Vaisberg
, A. Zavaleta

Universidad Peruana Cayetano Heredia

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The cytotoxic effect of venoms from six crotalinae Peruvian snakes (Bothrops atrox; B. brazili; B. pictus; B. barnetti; Lachesis m. muta y Crotalus durissus terrificus) was studied in an in vitro system of BALB/c 3T3 fibroblasts grown in Dulbecco modified minimal essential medium at 37 degrees C in a humidified atmosphere of 5% CO2-95% air. The viability of the cells was evaluated 24 hours after the treatment with the different venoms, using the method of exclusion of trypan blue. The six venoms produced cytotoxic effects at 24 hours on the 3T3 fibroblasts. The venom from B. atrox was the most potent (DE50 = 162 ng/ml) and that from B. barnetti the least (DE50 = 7182 ng/ml).

Translated title of the contribution	Cytotoxicity induced by Peruvian snake venom on fibroblasts of mice
Original language	Spanish
Pages (from-to)	143-145
Number of pages	3
Journal	Revista de biología tropical
Volume	40
Issue number	1
State	Published - Apr 1992

Cite this

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title = "Citotoxicidad inducida por veneno de serpientes peruanas sobre fibroblastos de rat{\'o}n.",

abstract = "The cytotoxic effect of venoms from six crotalinae Peruvian snakes (Bothrops atrox; B. brazili; B. pictus; B. barnetti; Lachesis m. muta y Crotalus durissus terrificus) was studied in an in vitro system of BALB/c 3T3 fibroblasts grown in Dulbecco modified minimal essential medium at 37 degrees C in a humidified atmosphere of 5\% CO2-95\% air. The viability of the cells was evaluated 24 hours after the treatment with the different venoms, using the method of exclusion of trypan blue. The six venoms produced cytotoxic effects at 24 hours on the 3T3 fibroblasts. The venom from B. atrox was the most potent (DE50 = 162 ng/ml) and that from B. barnetti the least (DE50 = 7182 ng/ml).",

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AU - Zavaleta, A.

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Y1 - 1992/4

N2 - The cytotoxic effect of venoms from six crotalinae Peruvian snakes (Bothrops atrox; B. brazili; B. pictus; B. barnetti; Lachesis m. muta y Crotalus durissus terrificus) was studied in an in vitro system of BALB/c 3T3 fibroblasts grown in Dulbecco modified minimal essential medium at 37 degrees C in a humidified atmosphere of 5% CO2-95% air. The viability of the cells was evaluated 24 hours after the treatment with the different venoms, using the method of exclusion of trypan blue. The six venoms produced cytotoxic effects at 24 hours on the 3T3 fibroblasts. The venom from B. atrox was the most potent (DE50 = 162 ng/ml) and that from B. barnetti the least (DE50 = 7182 ng/ml).

AB - The cytotoxic effect of venoms from six crotalinae Peruvian snakes (Bothrops atrox; B. brazili; B. pictus; B. barnetti; Lachesis m. muta y Crotalus durissus terrificus) was studied in an in vitro system of BALB/c 3T3 fibroblasts grown in Dulbecco modified minimal essential medium at 37 degrees C in a humidified atmosphere of 5% CO2-95% air. The viability of the cells was evaluated 24 hours after the treatment with the different venoms, using the method of exclusion of trypan blue. The six venoms produced cytotoxic effects at 24 hours on the 3T3 fibroblasts. The venom from B. atrox was the most potent (DE50 = 162 ng/ml) and that from B. barnetti the least (DE50 = 7182 ng/ml).

UR - https://www.scopus.com/pages/publications/0026849688

M3 - Artículo

C2 - 1297169

AN - SCOPUS:0026849688

SN - 0034-7744

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EP - 145

JO - Revista de biología tropical

JF - Revista de biología tropical

IS - 1

ER -

Citotoxicidad inducida por veneno de serpientes peruanas sobre fibroblastos de ratón.

Abstract

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