Comparison of three ELISAs for the routine diagnosis of human T-lymphotropic virus infection in a high-prevalence setting in Peru

Kristien Verdonck; Elsa González; Fernando Maldonado; Doris Agapito; Sonia Van Dooren; Anne Mieke Vandamme; Alfonso Silva-Santisteban; Guido Vanham; Daniel Clark; Eduardo Gotuzzo

doi:10.1016/j.trstmh.2008.12.002

Comparison of three ELISAs for the routine diagnosis of human T-lymphotropic virus infection in a high-prevalence setting in Peru

Kristien Verdonck
, Elsa González
, Fernando Maldonado
, Doris Agapito
, Sonia Van Dooren
, Anne Mieke Vandamme
, Alfonso Silva-Santisteban
, Guido Vanham
, Daniel Clark
, Eduardo Gotuzzo

Universidad Peruana Cayetano Heredia, Instituto de Medicina Tropical Alexander von Humboldt
Institute of Tropical Medicine
Universidad Peruana Cayetano Heredia
Katholieke Universiteit Leuven
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences
Hospital Nacional Cayetano Heredia

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

To compare three human T-lymphotropic virus (HTLV) ELISAs in a high-prevalence setting, we recruited 300 adults: 125 relatives of HTLV-infected subjects and 175 patients with possible diagnoses of HTLV-associated diseases. Sera were tested with Platelia, Murex, and Ortho ELISA. Samples with positive or discordant ELISA underwent confirmatory Inno-Lia testing. Inno-Lia gave 85/300 HTLV-1-positive and 1/300 HTLV-2-positive results. The positive predictive value was 98% for Platelia, and 100% for Murex and Ortho. Six samples had discordant ELISA; Murex gave one false-negative result, Ortho two and Platelia one. In high-prevalence settings, it is recommended to test samples with two ELISAs before considering them HTLV-seronegative.

Original language	English
Pages (from-to)	420-422
Number of pages	3
Journal	Transactions of the Royal Society of Tropical Medicine and Hygiene
Volume	103
Issue number	4
DOIs	https://doi.org/10.1016/j.trstmh.2008.12.002
State	Published - Apr 2009

Keywords

Diagnosis
ELISA
Human T-lymphotropic virus 1
Human T-lymphotropic virus 2
Peru
Predictive value of tests

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10.1016/j.trstmh.2008.12.002

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Verdonck, K., González, E., Maldonado, F., Agapito, D., Van Dooren, S., Vandamme, A. M., Silva-Santisteban, A., Vanham, G., Clark, D., & Gotuzzo, E. (2009). Comparison of three ELISAs for the routine diagnosis of human T-lymphotropic virus infection in a high-prevalence setting in Peru. Transactions of the Royal Society of Tropical Medicine and Hygiene, 103(4), 420-422. https://doi.org/10.1016/j.trstmh.2008.12.002

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title = "Comparison of three ELISAs for the routine diagnosis of human T-lymphotropic virus infection in a high-prevalence setting in Peru",

abstract = "To compare three human T-lymphotropic virus (HTLV) ELISAs in a high-prevalence setting, we recruited 300 adults: 125 relatives of HTLV-infected subjects and 175 patients with possible diagnoses of HTLV-associated diseases. Sera were tested with Platelia, Murex, and Ortho ELISA. Samples with positive or discordant ELISA underwent confirmatory Inno-Lia testing. Inno-Lia gave 85/300 HTLV-1-positive and 1/300 HTLV-2-positive results. The positive predictive value was 98\% for Platelia, and 100\% for Murex and Ortho. Six samples had discordant ELISA; Murex gave one false-negative result, Ortho two and Platelia one. In high-prevalence settings, it is recommended to test samples with two ELISAs before considering them HTLV-seronegative.",

keywords = "Diagnosis, ELISA, Human T-lymphotropic virus 1, Human T-lymphotropic virus 2, Peru, Predictive value of tests",

author = "Kristien Verdonck and Elsa Gonz{\'a}lez and Fernando Maldonado and Doris Agapito and \{Van Dooren\}, Sonia and Vandamme, \{Anne Mieke\} and Alfonso Silva-Santisteban and Guido Vanham and Daniel Clark and Eduardo Gotuzzo",

year = "2009",

month = apr,

doi = "10.1016/j.trstmh.2008.12.002",

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Verdonck, K, González, E, Maldonado, F, Agapito, D, Van Dooren, S, Vandamme, AM, Silva-Santisteban, A, Vanham, G, Clark, D & Gotuzzo, E 2009, 'Comparison of three ELISAs for the routine diagnosis of human T-lymphotropic virus infection in a high-prevalence setting in Peru', Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 103, no. 4, pp. 420-422. https://doi.org/10.1016/j.trstmh.2008.12.002

Comparison of three ELISAs for the routine diagnosis of human T-lymphotropic virus infection in a high-prevalence setting in Peru. / Verdonck, Kristien; González, Elsa; Maldonado, Fernando et al.
In: Transactions of the Royal Society of Tropical Medicine and Hygiene, Vol. 103, No. 4, 04.2009, p. 420-422.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Comparison of three ELISAs for the routine diagnosis of human T-lymphotropic virus infection in a high-prevalence setting in Peru

AU - Verdonck, Kristien

AU - González, Elsa

AU - Maldonado, Fernando

AU - Agapito, Doris

AU - Van Dooren, Sonia

AU - Vandamme, Anne Mieke

AU - Silva-Santisteban, Alfonso

AU - Vanham, Guido

AU - Clark, Daniel

AU - Gotuzzo, Eduardo

PY - 2009/4

Y1 - 2009/4

N2 - To compare three human T-lymphotropic virus (HTLV) ELISAs in a high-prevalence setting, we recruited 300 adults: 125 relatives of HTLV-infected subjects and 175 patients with possible diagnoses of HTLV-associated diseases. Sera were tested with Platelia, Murex, and Ortho ELISA. Samples with positive or discordant ELISA underwent confirmatory Inno-Lia testing. Inno-Lia gave 85/300 HTLV-1-positive and 1/300 HTLV-2-positive results. The positive predictive value was 98% for Platelia, and 100% for Murex and Ortho. Six samples had discordant ELISA; Murex gave one false-negative result, Ortho two and Platelia one. In high-prevalence settings, it is recommended to test samples with two ELISAs before considering them HTLV-seronegative.

AB - To compare three human T-lymphotropic virus (HTLV) ELISAs in a high-prevalence setting, we recruited 300 adults: 125 relatives of HTLV-infected subjects and 175 patients with possible diagnoses of HTLV-associated diseases. Sera were tested with Platelia, Murex, and Ortho ELISA. Samples with positive or discordant ELISA underwent confirmatory Inno-Lia testing. Inno-Lia gave 85/300 HTLV-1-positive and 1/300 HTLV-2-positive results. The positive predictive value was 98% for Platelia, and 100% for Murex and Ortho. Six samples had discordant ELISA; Murex gave one false-negative result, Ortho two and Platelia one. In high-prevalence settings, it is recommended to test samples with two ELISAs before considering them HTLV-seronegative.

KW - Diagnosis

KW - ELISA

KW - Human T-lymphotropic virus 1

KW - Human T-lymphotropic virus 2

KW - Peru

KW - Predictive value of tests

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DO - 10.1016/j.trstmh.2008.12.002

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AN - SCOPUS:61449267420

SN - 0035-9203

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SP - 420

EP - 422

JO - Transactions of the Royal Society of Tropical Medicine and Hygiene

JF - Transactions of the Royal Society of Tropical Medicine and Hygiene

IS - 4

ER -

Comparison of three ELISAs for the routine diagnosis of human T-lymphotropic virus infection in a high-prevalence setting in Peru

Abstract

Keywords

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