Culture-Independent Species Typing of Neotropical Leishmania for Clinical Validation of a PCR-Based Assay Targeting Heat Shock Protein 70 Genes

Lineth Garcia; Ann Kindt; Hernan Bermudez; Alejandro Llanos-Cuentas; Simonne De Doncker; Jorge Arevalo; Kelly Wilber Quispe Tintaya; Jean Claude Dujardin

doi:10.1128/JCM.42.5.2294-2297.2004

Culture-Independent Species Typing of Neotropical Leishmania for Clinical Validation of a PCR-Based Assay Targeting Heat Shock Protein 70 Genes

Lineth Garcia
, Ann Kindt
, Hernan Bermudez
, Alejandro Llanos-Cuentas
, Simonne De Doncker
, Jorge Arevalo
, Kelly Wilber Quispe Tintaya
, Jean Claude Dujardin

Institute of Tropical Medicine
Ctro. Univ. de Medicina Tropical
Universidad Peruana Cayetano Heredia, Instituto de Medicina Tropical Alexander von Humboldt

Research output: Contribution to journal › Article › peer-review

179 Scopus citations

Abstract

PCR-restriction fragment length polymorphism analysis of heat shock protein 70 genes discriminates most neotropical Leishmania species, as well as Trypanosoma cruzi. The assay, combined with capillary electrophoresis in a microchip device, may be applied directly on clinical samples with a high sensitivity, hence supporting clinical and epidemiological monitoring of leishmaniasis.

Original language	English
Pages (from-to)	2294-2297
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	42
Issue number	5
DOIs	https://doi.org/10.1128/JCM.42.5.2294-2297.2004
State	Published - May 2004

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Garcia, L., Kindt, A., Bermudez, H., Llanos-Cuentas, A., De Doncker, S., Arevalo, J., Tintaya, K. W. Q., & Dujardin, J. C. (2004). Culture-Independent Species Typing of Neotropical Leishmania for Clinical Validation of a PCR-Based Assay Targeting Heat Shock Protein 70 Genes. Journal of Clinical Microbiology, 42(5), 2294-2297. https://doi.org/10.1128/JCM.42.5.2294-2297.2004

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abstract = "PCR-restriction fragment length polymorphism analysis of heat shock protein 70 genes discriminates most neotropical Leishmania species, as well as Trypanosoma cruzi. The assay, combined with capillary electrophoresis in a microchip device, may be applied directly on clinical samples with a high sensitivity, hence supporting clinical and epidemiological monitoring of leishmaniasis.",

author = "Lineth Garcia and Ann Kindt and Hernan Bermudez and Alejandro Llanos-Cuentas and \{De Doncker\}, Simonne and Jorge Arevalo and Tintaya, \{Kelly Wilber Quispe\} and Dujardin, \{Jean Claude\}",

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Garcia, L, Kindt, A, Bermudez, H, Llanos-Cuentas, A, De Doncker, S, Arevalo, J, Tintaya, KWQ & Dujardin, JC 2004, 'Culture-Independent Species Typing of Neotropical Leishmania for Clinical Validation of a PCR-Based Assay Targeting Heat Shock Protein 70 Genes', Journal of Clinical Microbiology, vol. 42, no. 5, pp. 2294-2297. https://doi.org/10.1128/JCM.42.5.2294-2297.2004

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T1 - Culture-Independent Species Typing of Neotropical Leishmania for Clinical Validation of a PCR-Based Assay Targeting Heat Shock Protein 70 Genes

AU - Garcia, Lineth

AU - Kindt, Ann

AU - Bermudez, Hernan

AU - Llanos-Cuentas, Alejandro

AU - De Doncker, Simonne

AU - Arevalo, Jorge

AU - Tintaya, Kelly Wilber Quispe

AU - Dujardin, Jean Claude

PY - 2004/5

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N2 - PCR-restriction fragment length polymorphism analysis of heat shock protein 70 genes discriminates most neotropical Leishmania species, as well as Trypanosoma cruzi. The assay, combined with capillary electrophoresis in a microchip device, may be applied directly on clinical samples with a high sensitivity, hence supporting clinical and epidemiological monitoring of leishmaniasis.

AB - PCR-restriction fragment length polymorphism analysis of heat shock protein 70 genes discriminates most neotropical Leishmania species, as well as Trypanosoma cruzi. The assay, combined with capillary electrophoresis in a microchip device, may be applied directly on clinical samples with a high sensitivity, hence supporting clinical and epidemiological monitoring of leishmaniasis.

UR - https://www.scopus.com/pages/publications/2442551896

U2 - 10.1128/JCM.42.5.2294-2297.2004

DO - 10.1128/JCM.42.5.2294-2297.2004

M3 - Artículo

C2 - 15131217

AN - SCOPUS:2442551896

SN - 0095-1137

VL - 42

SP - 2294

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JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 5

ER -

Culture-Independent Species Typing of Neotropical Leishmania for Clinical Validation of a PCR-Based Assay Targeting Heat Shock Protein 70 Genes

Abstract

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