Desarrollo de un PCR para la Identificación del Parásito Dawestrema (Trematoda: Monogenea) en el Pez Arapaima gigas

Serrano Martínez Enrique; Tantaleán V. Manuel; Quispe H. Marco; Casas V. Gina; Londoñe B. Pablo

doi:10.15381/rivep.v27i3.11989

Desarrollo de un PCR para la Identificación del Parásito Dawestrema (Trematoda: Monogenea) en el Pez Arapaima gigas

Translated title of the contribution: Development of a PCR assay for the identification of the parasite dawestrema (Trematoda: Monogenea) in arapaima gigas fish

Serrano Martínez Enrique
, Tantaleán V. Manuel
, Quispe H. Marco
, Casas V. Gina
, Londoñe B. Pablo

Universidad Peruana Cayetano Heredia, Facultad de Medicina Alberto Hurtado
Instituto Tecnológico de la Producción, Callao

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Studies in the Amazonian species Arapaima gigas («paiche») have demonstrated the presence of Dawestrema cycloancistrium monogenean parasite in high parasite loads, mainly in stages between 180 and 365 days of age. D. cycloancistrium adheres to the gills causing a decrease in the degree of oxygenation and stress on the fish predisponing to disease presentation. This study aimed to develop a molecular diagnostic technique for D. cycloancistrium in order to aply it in the management of the fish biodiversity. For this purpose, 200 specimens of D. cycloancistrium were collected, DNA was extracted and quantified, primers were designed and a PCR was developed. The amplification of a sequence of 236 bp of the Dawestrema monogenean was obtained.

Translated title of the contribution	Development of a PCR assay for the identification of the parasite dawestrema (Trematoda: Monogenea) in arapaima gigas fish
Original language	Spanish
Pages (from-to)	581-588
Number of pages	8
Journal	Revista de Investigaciones Veterinarias del Peru
Volume	27
Issue number	3
DOIs	https://doi.org/10.15381/rivep.v27i3.11989
State	Published - 1 Jul 2016
Externally published	Yes

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10.15381/rivep.v27i3.11989

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title = "Desarrollo de un PCR para la Identificaci{\'o}n del Par{\'a}sito Dawestrema (Trematoda: Monogenea) en el Pez Arapaima gigas",

abstract = "Studies in the Amazonian species Arapaima gigas («paiche») have demonstrated the presence of Dawestrema cycloancistrium monogenean parasite in high parasite loads, mainly in stages between 180 and 365 days of age. D. cycloancistrium adheres to the gills causing a decrease in the degree of oxygenation and stress on the fish predisponing to disease presentation. This study aimed to develop a molecular diagnostic technique for D. cycloancistrium in order to aply it in the management of the fish biodiversity. For this purpose, 200 specimens of D. cycloancistrium were collected, DNA was extracted and quantified, primers were designed and a PCR was developed. The amplification of a sequence of 236 bp of the Dawestrema monogenean was obtained.",

keywords = "Aquatic health, Biodiversity, D. cycloancistrium, Molecular biology, PCR, Paiche, Parasites",

author = "Enrique, \{Serrano Mart{\'i}nez\} and Manuel, \{Tantale{\'a}n V.\} and Marco, \{Quispe H.\} and Gina, \{Casas V.\} and Pablo, \{Londo{\~n}e B.\}",

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doi = "10.15381/rivep.v27i3.11989",

language = "Espa{\~n}ol",

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pages = "581--588",

journal = "Revista de Investigaciones Veterinarias del Peru",

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TY - JOUR

T1 - Desarrollo de un PCR para la Identificación del Parásito Dawestrema (Trematoda: Monogenea) en el Pez Arapaima gigas

AU - Enrique, Serrano Martínez

AU - Manuel, Tantaleán V.

AU - Marco, Quispe H.

AU - Gina, Casas V.

AU - Pablo, Londoñe B.

PY - 2016/7/1

Y1 - 2016/7/1

N2 - Studies in the Amazonian species Arapaima gigas («paiche») have demonstrated the presence of Dawestrema cycloancistrium monogenean parasite in high parasite loads, mainly in stages between 180 and 365 days of age. D. cycloancistrium adheres to the gills causing a decrease in the degree of oxygenation and stress on the fish predisponing to disease presentation. This study aimed to develop a molecular diagnostic technique for D. cycloancistrium in order to aply it in the management of the fish biodiversity. For this purpose, 200 specimens of D. cycloancistrium were collected, DNA was extracted and quantified, primers were designed and a PCR was developed. The amplification of a sequence of 236 bp of the Dawestrema monogenean was obtained.

AB - Studies in the Amazonian species Arapaima gigas («paiche») have demonstrated the presence of Dawestrema cycloancistrium monogenean parasite in high parasite loads, mainly in stages between 180 and 365 days of age. D. cycloancistrium adheres to the gills causing a decrease in the degree of oxygenation and stress on the fish predisponing to disease presentation. This study aimed to develop a molecular diagnostic technique for D. cycloancistrium in order to aply it in the management of the fish biodiversity. For this purpose, 200 specimens of D. cycloancistrium were collected, DNA was extracted and quantified, primers were designed and a PCR was developed. The amplification of a sequence of 236 bp of the Dawestrema monogenean was obtained.

KW - Aquatic health

KW - Biodiversity

KW - D. cycloancistrium

KW - Molecular biology

KW - PCR

KW - Paiche

KW - Parasites

UR - https://www.scopus.com/pages/publications/85001930700

U2 - 10.15381/rivep.v27i3.11989

DO - 10.15381/rivep.v27i3.11989

M3 - Artículo

AN - SCOPUS:85001930700

SN - 1682-3419

VL - 27

SP - 581

EP - 588

JO - Revista de Investigaciones Veterinarias del Peru

JF - Revista de Investigaciones Veterinarias del Peru

IS - 3

ER -

Desarrollo de un PCR para la Identificación del Parásito Dawestrema (Trematoda: Monogenea) en el Pez Arapaima gigas

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