Detection of Mycobacterium Tuberculosis DNA in Buccal Swab Samples from Children in Lima, Peru

Juan A. Flores; Roger Calderón; Annelies W. Mesman; Martin Soto; Julia Coit; Juan Aliaga; Milagros Mendoza; Segundo R. Leon; Kelika Konda; Francisco M. Mestanza; Carlos J. Mendoza; Leonid Lecca; Megan B. Murray; Rebecca C. Holmberg; Nira R. Pollock; Molly F. Franke

doi:10.1097/INF.0000000000002828

Detection of Mycobacterium Tuberculosis DNA in Buccal Swab Samples from Children in Lima, Peru

Juan A. Flores
, Roger Calderón
, Annelies W. Mesman
, Martin Soto
, Julia Coit
, Juan Aliaga
, Milagros Mendoza
, Segundo R. Leon
, Kelika Konda
, Francisco M. Mestanza
, Carlos J. Mendoza
, Leonid Lecca
, Megan B. Murray
, Rebecca C. Holmberg
, Nira R. Pollock
, Molly F. Franke

Center for Interdisciplinary Research on Sexuality, AIDS, and Society

San Juan Bautista Private University
Universidad Peruana Cayetano Heredia
Socios en Salud Lima
Universidade Federal do Rio de Janeiro
Harvard Medical School
San Bartolomé Mother-Child National Teaching Hospital
Ministry of Health of Peru
Akonni Biosystems Inc
Dana-Farber Cancer Institute

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

We examined Mycobacterium tuberculosis DNA detection from buccal swab samples collected from children in Lima, Peru. DNA was extracted and amplified via real-time polymerase chain reaction. Sensitivity was 21% (95% confidence interval [CI]: 7%-42%) in 24 culture-confirmed tuberculosis cases and 4.6% (95% CI: 1%-13%) in 65 clinically diagnosed unconfirmed cases. Sensitivity was highest for smear-positive tuberculosis. Specificity was 99% in the 199 controls (95% CI: 96%-100%).

Original language	English
Pages (from-to)	E376-E380
Journal	Pediatric Infectious Disease Journal
Volume	39
Issue number	11
DOIs	https://doi.org/10.1097/INF.0000000000002828
State	Published - 1 Nov 2020

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

childhood tuberculosis
diagnostic
oral swab
real-time polymerase chain reaction
tuberculosis

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10.1097/INF.0000000000002828

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Flores, J. A., Calderón, R., Mesman, A. W., Soto, M., Coit, J., Aliaga, J., Mendoza, M., Leon, S. R., Konda, K., Mestanza, F. M., Mendoza, C. J., Lecca, L., Murray, M. B., Holmberg, R. C., Pollock, N. R., & Franke, M. F. (2020). Detection of Mycobacterium Tuberculosis DNA in Buccal Swab Samples from Children in Lima, Peru. Pediatric Infectious Disease Journal, 39(11), E376-E380. https://doi.org/10.1097/INF.0000000000002828

@article{26e564298d9b44d687aef3a01c48db97,

title = "Detection of Mycobacterium Tuberculosis DNA in Buccal Swab Samples from Children in Lima, Peru",

abstract = "We examined Mycobacterium tuberculosis DNA detection from buccal swab samples collected from children in Lima, Peru. DNA was extracted and amplified via real-time polymerase chain reaction. Sensitivity was 21\% (95\% confidence interval [CI]: 7\%-42\%) in 24 culture-confirmed tuberculosis cases and 4.6\% (95\% CI: 1\%-13\%) in 65 clinically diagnosed unconfirmed cases. Sensitivity was highest for smear-positive tuberculosis. Specificity was 99\% in the 199 controls (95\% CI: 96\%-100\%).",

keywords = "childhood tuberculosis, diagnostic, oral swab, real-time polymerase chain reaction, tuberculosis",

author = "Flores, \{Juan A.\} and Roger Calder{\'o}n and Mesman, \{Annelies W.\} and Martin Soto and Julia Coit and Juan Aliaga and Milagros Mendoza and Leon, \{Segundo R.\} and Kelika Konda and Mestanza, \{Francisco M.\} and Mendoza, \{Carlos J.\} and Leonid Lecca and Murray, \{Megan B.\} and Holmberg, \{Rebecca C.\} and Pollock, \{Nira R.\} and Franke, \{Molly F.\}",

year = "2020",

month = nov,

day = "1",

doi = "10.1097/INF.0000000000002828",

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Flores, JA, Calderón, R, Mesman, AW, Soto, M, Coit, J, Aliaga, J, Mendoza, M, Leon, SR, Konda, K, Mestanza, FM, Mendoza, CJ, Lecca, L, Murray, MB, Holmberg, RC, Pollock, NR & Franke, MF 2020, 'Detection of Mycobacterium Tuberculosis DNA in Buccal Swab Samples from Children in Lima, Peru', Pediatric Infectious Disease Journal, vol. 39, no. 11, pp. E376-E380. https://doi.org/10.1097/INF.0000000000002828

TY - JOUR

T1 - Detection of Mycobacterium Tuberculosis DNA in Buccal Swab Samples from Children in Lima, Peru

AU - Flores, Juan A.

AU - Calderón, Roger

AU - Mesman, Annelies W.

AU - Soto, Martin

AU - Coit, Julia

AU - Aliaga, Juan

AU - Mendoza, Milagros

AU - Leon, Segundo R.

AU - Konda, Kelika

AU - Mestanza, Francisco M.

AU - Mendoza, Carlos J.

AU - Lecca, Leonid

AU - Murray, Megan B.

AU - Holmberg, Rebecca C.

AU - Pollock, Nira R.

AU - Franke, Molly F.

PY - 2020/11/1

Y1 - 2020/11/1

N2 - We examined Mycobacterium tuberculosis DNA detection from buccal swab samples collected from children in Lima, Peru. DNA was extracted and amplified via real-time polymerase chain reaction. Sensitivity was 21% (95% confidence interval [CI]: 7%-42%) in 24 culture-confirmed tuberculosis cases and 4.6% (95% CI: 1%-13%) in 65 clinically diagnosed unconfirmed cases. Sensitivity was highest for smear-positive tuberculosis. Specificity was 99% in the 199 controls (95% CI: 96%-100%).

AB - We examined Mycobacterium tuberculosis DNA detection from buccal swab samples collected from children in Lima, Peru. DNA was extracted and amplified via real-time polymerase chain reaction. Sensitivity was 21% (95% confidence interval [CI]: 7%-42%) in 24 culture-confirmed tuberculosis cases and 4.6% (95% CI: 1%-13%) in 65 clinically diagnosed unconfirmed cases. Sensitivity was highest for smear-positive tuberculosis. Specificity was 99% in the 199 controls (95% CI: 96%-100%).

KW - childhood tuberculosis

KW - diagnostic

KW - oral swab

KW - real-time polymerase chain reaction

KW - tuberculosis

UR - https://www.scopus.com/pages/publications/85093908062

U2 - 10.1097/INF.0000000000002828

DO - 10.1097/INF.0000000000002828

M3 - Artículo

C2 - 32675773

AN - SCOPUS:85093908062

SN - 0891-3668

VL - 39

SP - E376-E380

JO - Pediatric Infectious Disease Journal

JF - Pediatric Infectious Disease Journal

IS - 11

ER -

Detection of Mycobacterium Tuberculosis DNA in Buccal Swab Samples from Children in Lima, Peru

Abstract

UN SDGs

Keywords

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