Development and evaluation of porcine cysticercosis quickelisa ™ in triturus® EIA analyzer

Sukwan Handali; Sowmya Pattabhi; Yeuk Mui Lee; Maria Silva-Ibanez; Victor A. Kovalenko; Andrew E. Levin; Armando E. Gonzalez; Jacquelin M. Roberts; Hector H. Garcia; Robert H. Gilman; Kathy Hancock; Victor C.W. Tsang

doi:10.1080/15321810903405068

Development and evaluation of porcine cysticercosis quickelisa ^™ in triturus^® EIA analyzer

Sukwan Handali
, Sowmya Pattabhi
, Yeuk Mui Lee
, Maria Silva-Ibanez
, Victor A. Kovalenko
, Andrew E. Levin
, Armando E. Gonzalez
, Jacquelin M. Roberts
, Hector H. Garcia
, Robert H. Gilman
, Kathy Hancock
, Victor C.W. Tsang

School of Science and Engineering

Atlanta Research and Education Foundation
Centers for Disease Control and Prevention
Immunetics Inc.
Univ. Nacional Mayor de San Marcos
Instituto Nacional de Ciencias Neurológicas
Johns Hopkins Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

We evaluated three diagnostic antigens (recombinant GP50, recombinant T24H, and synthetic Ts18var1) for cysticercosis and found that all three performed well in detecting cysticercosis in humans and pigs in several assay formats. These antigens were adapted to a new antibody detection format (QuickELISA). With one single incubation step which involves all reactants except the enzyme substrate, the QuickELISA is particularly suited for automation. We formatted the QuickELISA for the Triturus EIA analyzer for testing large numbers of samples. We found that in QuickELISA formats rGP50 and rT24H have better sensitivity and specificity than sTs18var1 for detecting porcine cysticercosis.

Original language	English
Pages (from-to)	60-70
Number of pages	11
Journal	Journal of Immunoassay and Immunochemistry
Volume	31
Issue number	1
DOIs	https://doi.org/10.1080/15321810903405068
State	Published - Jan 2010

Keywords

Assay development
Cysticercosis
ELISA
Pig
Serological diagnosis
Taenia solium

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10.1080/15321810903405068

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Handali, S., Pattabhi, S., Lee, Y. M., Silva-Ibanez, M., Kovalenko, V. A., Levin, A. E., Gonzalez, A. E., Roberts, J. M., Garcia, H. H., Gilman, R. H., Hancock, K., & Tsang, V. C. W. (2010). Development and evaluation of porcine cysticercosis quickelisa ^™ in triturus^® EIA analyzer. Journal of Immunoassay and Immunochemistry, 31(1), 60-70. https://doi.org/10.1080/15321810903405068

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title = "Development and evaluation of porcine cysticercosis quickelisa {\texttrademark} in triturus{\textregistered} EIA analyzer",

abstract = "We evaluated three diagnostic antigens (recombinant GP50, recombinant T24H, and synthetic Ts18var1) for cysticercosis and found that all three performed well in detecting cysticercosis in humans and pigs in several assay formats. These antigens were adapted to a new antibody detection format (QuickELISA). With one single incubation step which involves all reactants except the enzyme substrate, the QuickELISA is particularly suited for automation. We formatted the QuickELISA for the Triturus EIA analyzer for testing large numbers of samples. We found that in QuickELISA formats rGP50 and rT24H have better sensitivity and specificity than sTs18var1 for detecting porcine cysticercosis.",

keywords = "Assay development, Cysticercosis, ELISA, Pig, Serological diagnosis, Taenia solium",

author = "Sukwan Handali and Sowmya Pattabhi and Lee, \{Yeuk Mui\} and Maria Silva-Ibanez and Kovalenko, \{Victor A.\} and Levin, \{Andrew E.\} and Gonzalez, \{Armando E.\} and Roberts, \{Jacquelin M.\} and Garcia, \{Hector H.\} and Gilman, \{Robert H.\} and Kathy Hancock and Tsang, \{Victor C.W.\}",

year = "2010",

month = jan,

doi = "10.1080/15321810903405068",

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T1 - Development and evaluation of porcine cysticercosis quickelisa ™ in triturus® EIA analyzer

AU - Handali, Sukwan

AU - Pattabhi, Sowmya

AU - Lee, Yeuk Mui

AU - Silva-Ibanez, Maria

AU - Kovalenko, Victor A.

AU - Levin, Andrew E.

AU - Gonzalez, Armando E.

AU - Roberts, Jacquelin M.

AU - Garcia, Hector H.

AU - Gilman, Robert H.

AU - Hancock, Kathy

AU - Tsang, Victor C.W.

PY - 2010/1

Y1 - 2010/1

N2 - We evaluated three diagnostic antigens (recombinant GP50, recombinant T24H, and synthetic Ts18var1) for cysticercosis and found that all three performed well in detecting cysticercosis in humans and pigs in several assay formats. These antigens were adapted to a new antibody detection format (QuickELISA). With one single incubation step which involves all reactants except the enzyme substrate, the QuickELISA is particularly suited for automation. We formatted the QuickELISA for the Triturus EIA analyzer for testing large numbers of samples. We found that in QuickELISA formats rGP50 and rT24H have better sensitivity and specificity than sTs18var1 for detecting porcine cysticercosis.

AB - We evaluated three diagnostic antigens (recombinant GP50, recombinant T24H, and synthetic Ts18var1) for cysticercosis and found that all three performed well in detecting cysticercosis in humans and pigs in several assay formats. These antigens were adapted to a new antibody detection format (QuickELISA). With one single incubation step which involves all reactants except the enzyme substrate, the QuickELISA is particularly suited for automation. We formatted the QuickELISA for the Triturus EIA analyzer for testing large numbers of samples. We found that in QuickELISA formats rGP50 and rT24H have better sensitivity and specificity than sTs18var1 for detecting porcine cysticercosis.

KW - Assay development

KW - Cysticercosis

KW - ELISA

KW - Pig

KW - Serological diagnosis

KW - Taenia solium

UR - https://www.scopus.com/pages/publications/77951244786

U2 - 10.1080/15321810903405068

DO - 10.1080/15321810903405068

M3 - Artículo

C2 - 20391018

AN - SCOPUS:77951244786

SN - 1532-1819

VL - 31

SP - 60

EP - 70

JO - Journal of Immunoassay and Immunochemistry

JF - Journal of Immunoassay and Immunochemistry

IS - 1

ER -

Development and evaluation of porcine cysticercosis quickelisa ^™ in triturus^® EIA analyzer

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Keywords

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