Genetic variation in KRTAP11.1 gene of Suri alpaca (Vicugna pacos) from Puno, Peru

Roberto Gallegos; Jorge Rodriguez; Ivan Quiñones; Irene Delgado-De-la-Flor; Teodosio Huanca; Abigail de la Cruz Perez; José R. Espinoza

doi:10.34044/j.anres.2019.53.1.15

Genetic variation in KRTAP11.1 gene of Suri alpaca (Vicugna pacos) from Puno, Peru

Roberto Gallegos
, Jorge Rodriguez
, Ivan Quiñones
, Irene Delgado-De-la-Flor
, Teodosio Huanca
, Abigail de la Cruz Perez
, José R. Espinoza

School of Science and Engineering

Universidad Nacional del Altiplano
Universidad Peruana Cayetano Heredia
National Institute of Agrarian Innovation

Research output: Contribution to journal › Article › peer-review

Abstract

The objective of this study was to identify genetic single nucleotide polymorphisms (SNPs) in the coding region of the KRTAP11.1 gene from the Suri alpaca population from Puno, Peru. Genomic DNA (n = 119) was used to amplify the 636 bp fragment of the coding region from the KRTAP11.1 gene. The polymerase chain reaction products of the KRTAP11.1 gene were sequenced by both strands and polymorphism type SNPs were identified. All SNPs (n = 3) were in Hardy-Weinberg equilibrium and had a high level of genotypic linkage disequilibrium with the presence of two haplotypes. All genetic polymorphisms generate non-synonymous amino acids changes: SNP 110 T>G (Ser>Ala), SNP 363 T>G (Phe>Cys) and SNP 375 A>C (Tyr>Ser). The results indicated the presence of moderate genetic diversity in the coding region of the KRTAP11.1 gene from the Suri alpaca population.

Original language	English
Pages (from-to)	87-88
Number of pages	2
Journal	Agriculture and Natural Resources
Volume	53
Issue number	1
DOIs	https://doi.org/10.34044/j.anres.2019.53.1.15
State	Published - 2019

Keywords

Alpaca
KAP11.1 gene
Keratin associated protein
Single nucleotide polymorphisms (SNPs)
Suri

Access to Document

10.34044/j.anres.2019.53.1.15

Cite this

@article{c2d795d0057f40139a097ee82c24ea60,

title = "Genetic variation in KRTAP11.1 gene of Suri alpaca (Vicugna pacos) from Puno, Peru",

abstract = "The objective of this study was to identify genetic single nucleotide polymorphisms (SNPs) in the coding region of the KRTAP11.1 gene from the Suri alpaca population from Puno, Peru. Genomic DNA (n = 119) was used to amplify the 636 bp fragment of the coding region from the KRTAP11.1 gene. The polymerase chain reaction products of the KRTAP11.1 gene were sequenced by both strands and polymorphism type SNPs were identified. All SNPs (n = 3) were in Hardy-Weinberg equilibrium and had a high level of genotypic linkage disequilibrium with the presence of two haplotypes. All genetic polymorphisms generate non-synonymous amino acids changes: SNP 110 T>G (Ser>Ala), SNP 363 T>G (Phe>Cys) and SNP 375 A>C (Tyr>Ser). The results indicated the presence of moderate genetic diversity in the coding region of the KRTAP11.1 gene from the Suri alpaca population.",

keywords = "Alpaca, KAP11.1 gene, Keratin associated protein, Single nucleotide polymorphisms (SNPs), Suri",

author = "Roberto Gallegos and Jorge Rodriguez and Ivan Qui{\~n}ones and Irene Delgado-De-la-Flor and Teodosio Huanca and \{de la Cruz Perez\}, Abigail and Espinoza, \{Jos{\'e} R.\}",

year = "2019",

doi = "10.34044/j.anres.2019.53.1.15",

language = "Ingl{\'e}s",

volume = "53",

pages = "87--88",

journal = "Agriculture and Natural Resources",

issn = "2468-1458",

number = "1",

}

TY - JOUR

T1 - Genetic variation in KRTAP11.1 gene of Suri alpaca (Vicugna pacos) from Puno, Peru

AU - Gallegos, Roberto

AU - Rodriguez, Jorge

AU - Quiñones, Ivan

AU - Delgado-De-la-Flor, Irene

AU - Huanca, Teodosio

AU - de la Cruz Perez, Abigail

AU - Espinoza, José R.

PY - 2019

Y1 - 2019

N2 - The objective of this study was to identify genetic single nucleotide polymorphisms (SNPs) in the coding region of the KRTAP11.1 gene from the Suri alpaca population from Puno, Peru. Genomic DNA (n = 119) was used to amplify the 636 bp fragment of the coding region from the KRTAP11.1 gene. The polymerase chain reaction products of the KRTAP11.1 gene were sequenced by both strands and polymorphism type SNPs were identified. All SNPs (n = 3) were in Hardy-Weinberg equilibrium and had a high level of genotypic linkage disequilibrium with the presence of two haplotypes. All genetic polymorphisms generate non-synonymous amino acids changes: SNP 110 T>G (Ser>Ala), SNP 363 T>G (Phe>Cys) and SNP 375 A>C (Tyr>Ser). The results indicated the presence of moderate genetic diversity in the coding region of the KRTAP11.1 gene from the Suri alpaca population.

AB - The objective of this study was to identify genetic single nucleotide polymorphisms (SNPs) in the coding region of the KRTAP11.1 gene from the Suri alpaca population from Puno, Peru. Genomic DNA (n = 119) was used to amplify the 636 bp fragment of the coding region from the KRTAP11.1 gene. The polymerase chain reaction products of the KRTAP11.1 gene were sequenced by both strands and polymorphism type SNPs were identified. All SNPs (n = 3) were in Hardy-Weinberg equilibrium and had a high level of genotypic linkage disequilibrium with the presence of two haplotypes. All genetic polymorphisms generate non-synonymous amino acids changes: SNP 110 T>G (Ser>Ala), SNP 363 T>G (Phe>Cys) and SNP 375 A>C (Tyr>Ser). The results indicated the presence of moderate genetic diversity in the coding region of the KRTAP11.1 gene from the Suri alpaca population.

KW - Alpaca

KW - KAP11.1 gene

KW - Keratin associated protein

KW - Single nucleotide polymorphisms (SNPs)

KW - Suri

UR - https://www.scopus.com/pages/publications/85072796597

U2 - 10.34044/j.anres.2019.53.1.15

DO - 10.34044/j.anres.2019.53.1.15

M3 - Artículo

AN - SCOPUS:85072796597

SN - 2468-1458

VL - 53

SP - 87

EP - 88

JO - Agriculture and Natural Resources

JF - Agriculture and Natural Resources

IS - 1

ER -

Genetic variation in KRTAP11.1 gene of Suri alpaca (Vicugna pacos) from Puno, Peru

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this