High degree of Plasmodium vivax diversity in the peruvian amazon demonstrated by tandem repeat polymorphism analysis

Margaret Kosek; Pablo P. Yori; Robert H. Gilman; Maritza Calderon; Mirko Zimic; Raul Chuquiyauri; Cesar Jeri; Viviana Pinedo-Cancino; Michael A. Matthias; Alejandro Llanos-Cuentas; Joseph M. Vinetz

doi:10.4269/ajtmh.2012.11-0627

High degree of Plasmodium vivax diversity in the peruvian amazon demonstrated by tandem repeat polymorphism analysis

Margaret Kosek
, Pablo P. Yori
, Robert H. Gilman
, Maritza Calderon
, Mirko Zimic
, Raul Chuquiyauri
, Cesar Jeri
, Viviana Pinedo-Cancino
, Michael A. Matthias
, Alejandro Llanos-Cuentas
, Joseph M. Vinetz

Johns Hopkins Bloomberg School of Public Health
Asociación Benéfica PRISMA
Universidad Peruana Cayetano Heredia
Department of Medicine

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Molecular tools to distinguish strains of Plasmodium vivax are important for studying the epidemiology of malaria transmission. Two sets of markers - tandem repeat (TR) polymorphisms and MSP3α - were used to study Plasmodium vivax in patients in the Peruvian Amazon region of Iquitos. Of 110 patients, 90 distinct haplotypes were distinguished using 9 TR markers. An MSP3α polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using HhaI and AluI revealed 8 and 9 profiles, respectively, and 36 profiles when analyzed in combination. Combining TR and PCR-RFLP markers, 101 distinct molecular profiles were distinguished among these 110 patients. Nine TR markers arrayed along a 100 kB stretch of a P. vivax chromosome containing the gene for circumsporozoite protein showed non-linear linkage disequilibrium (I _SA = 0.03, P = 0.001). These findings demonstrate the potential use of TR markers for molecular epidemiology studies.

Original language	English
Pages (from-to)	580-586
Number of pages	7
Journal	American Journal of Tropical Medicine and Hygiene
Volume	86
Issue number	4
DOIs	https://doi.org/10.4269/ajtmh.2012.11-0627
State	Published - Apr 2012

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Kosek, M., Yori, P. P., Gilman, R. H., Calderon, M., Zimic, M., Chuquiyauri, R., Jeri, C., Pinedo-Cancino, V., Matthias, M. A., Llanos-Cuentas, A., & Vinetz, J. M. (2012). High degree of Plasmodium vivax diversity in the peruvian amazon demonstrated by tandem repeat polymorphism analysis. American Journal of Tropical Medicine and Hygiene, 86(4), 580-586. https://doi.org/10.4269/ajtmh.2012.11-0627

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title = "High degree of Plasmodium vivax diversity in the peruvian amazon demonstrated by tandem repeat polymorphism analysis",

abstract = "Molecular tools to distinguish strains of Plasmodium vivax are important for studying the epidemiology of malaria transmission. Two sets of markers - tandem repeat (TR) polymorphisms and MSP3α - were used to study Plasmodium vivax in patients in the Peruvian Amazon region of Iquitos. Of 110 patients, 90 distinct haplotypes were distinguished using 9 TR markers. An MSP3α polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using HhaI and AluI revealed 8 and 9 profiles, respectively, and 36 profiles when analyzed in combination. Combining TR and PCR-RFLP markers, 101 distinct molecular profiles were distinguished among these 110 patients. Nine TR markers arrayed along a 100 kB stretch of a P. vivax chromosome containing the gene for circumsporozoite protein showed non-linear linkage disequilibrium (I SA = 0.03, P = 0.001). These findings demonstrate the potential use of TR markers for molecular epidemiology studies.",

author = "Margaret Kosek and Yori, \{Pablo P.\} and Gilman, \{Robert H.\} and Maritza Calderon and Mirko Zimic and Raul Chuquiyauri and Cesar Jeri and Viviana Pinedo-Cancino and Matthias, \{Michael A.\} and Alejandro Llanos-Cuentas and Vinetz, \{Joseph M.\}",

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doi = "10.4269/ajtmh.2012.11-0627",

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journal = "American Journal of Tropical Medicine and Hygiene",

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Kosek, M, Yori, PP, Gilman, RH, Calderon, M , Zimic, M, Chuquiyauri, R, Jeri, C, Pinedo-Cancino, V, Matthias, MA, Llanos-Cuentas, A & Vinetz, JM 2012, 'High degree of Plasmodium vivax diversity in the peruvian amazon demonstrated by tandem repeat polymorphism analysis', American Journal of Tropical Medicine and Hygiene, vol. 86, no. 4, pp. 580-586. https://doi.org/10.4269/ajtmh.2012.11-0627

TY - JOUR

T1 - High degree of Plasmodium vivax diversity in the peruvian amazon demonstrated by tandem repeat polymorphism analysis

AU - Kosek, Margaret

AU - Yori, Pablo P.

AU - Gilman, Robert H.

AU - Calderon, Maritza

AU - Zimic, Mirko

AU - Chuquiyauri, Raul

AU - Jeri, Cesar

AU - Pinedo-Cancino, Viviana

AU - Matthias, Michael A.

AU - Llanos-Cuentas, Alejandro

AU - Vinetz, Joseph M.

PY - 2012/4

Y1 - 2012/4

N2 - Molecular tools to distinguish strains of Plasmodium vivax are important for studying the epidemiology of malaria transmission. Two sets of markers - tandem repeat (TR) polymorphisms and MSP3α - were used to study Plasmodium vivax in patients in the Peruvian Amazon region of Iquitos. Of 110 patients, 90 distinct haplotypes were distinguished using 9 TR markers. An MSP3α polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using HhaI and AluI revealed 8 and 9 profiles, respectively, and 36 profiles when analyzed in combination. Combining TR and PCR-RFLP markers, 101 distinct molecular profiles were distinguished among these 110 patients. Nine TR markers arrayed along a 100 kB stretch of a P. vivax chromosome containing the gene for circumsporozoite protein showed non-linear linkage disequilibrium (I SA = 0.03, P = 0.001). These findings demonstrate the potential use of TR markers for molecular epidemiology studies.

AB - Molecular tools to distinguish strains of Plasmodium vivax are important for studying the epidemiology of malaria transmission. Two sets of markers - tandem repeat (TR) polymorphisms and MSP3α - were used to study Plasmodium vivax in patients in the Peruvian Amazon region of Iquitos. Of 110 patients, 90 distinct haplotypes were distinguished using 9 TR markers. An MSP3α polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using HhaI and AluI revealed 8 and 9 profiles, respectively, and 36 profiles when analyzed in combination. Combining TR and PCR-RFLP markers, 101 distinct molecular profiles were distinguished among these 110 patients. Nine TR markers arrayed along a 100 kB stretch of a P. vivax chromosome containing the gene for circumsporozoite protein showed non-linear linkage disequilibrium (I SA = 0.03, P = 0.001). These findings demonstrate the potential use of TR markers for molecular epidemiology studies.

UR - https://www.scopus.com/pages/publications/84860505028

U2 - 10.4269/ajtmh.2012.11-0627

DO - 10.4269/ajtmh.2012.11-0627

M3 - Artículo

C2 - 22492139

AN - SCOPUS:84860505028

SN - 0002-9637

VL - 86

SP - 580

EP - 586

JO - American Journal of Tropical Medicine and Hygiene

JF - American Journal of Tropical Medicine and Hygiene

IS - 4

ER -

High degree of Plasmodium vivax diversity in the peruvian amazon demonstrated by tandem repeat polymorphism analysis

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