Leishmania (Viannia) species identification on clinical samples from cutaneous leishmaniasis patients in Peru: Assessment of a molecular stepwise approach

Nicolas Veland; Andrea K. Boggild; Cristian Valencia; Braulio M. Valencia; Alejandro Llanos-Cuentas; Gert Van Der Auwera; Jean Claude Dujardin; Jorge Arevalo

doi:10.1128/JCM.05061-11

Leishmania (Viannia) species identification on clinical samples from cutaneous leishmaniasis patients in Peru: Assessment of a molecular stepwise approach

Nicolas Veland
, Andrea K. Boggild
, Cristian Valencia
, Braulio M. Valencia
, Alejandro Llanos-Cuentas
, Gert Van Der Auwera
, Jean Claude Dujardin
, Jorge Arevalo

Universidad Peruana Cayetano Heredia, Instituto de Medicina Tropical Alexander von Humboldt
Toronto General Hospital
Hospital Nacional Cayetano Heredia
Institute of Tropical Medicine
University of Antwerp

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Wepresent an algorithm based on three PCR assays for Leishmania (Viannia) species identification and assessed its performance using 70 specimens from Peruvian patients. The succession of the assayed targets can be ordered according to species prevalence. Sequential progression through the algorithm reduced the number of samples here studied by approximately 30% after each step.

Original language	English
Pages (from-to)	495-498
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	50
Issue number	2
DOIs	https://doi.org/10.1128/JCM.05061-11
State	Published - Feb 2012

Access to Document

10.1128/JCM.05061-11

Cite this

Veland, N., Boggild, A. K., Valencia, C., Valencia, B. M., Llanos-Cuentas, A., Van Der Auwera, G., Dujardin, J. C., & Arevalo, J. (2012). Leishmania (Viannia) species identification on clinical samples from cutaneous leishmaniasis patients in Peru: Assessment of a molecular stepwise approach. Journal of Clinical Microbiology, 50(2), 495-498. https://doi.org/10.1128/JCM.05061-11

@article{39bef278d5694674850347179f4484c3,

title = "Leishmania (Viannia) species identification on clinical samples from cutaneous leishmaniasis patients in Peru: Assessment of a molecular stepwise approach",

abstract = "Wepresent an algorithm based on three PCR assays for Leishmania (Viannia) species identification and assessed its performance using 70 specimens from Peruvian patients. The succession of the assayed targets can be ordered according to species prevalence. Sequential progression through the algorithm reduced the number of samples here studied by approximately 30\% after each step.",

author = "Nicolas Veland and Boggild, \{Andrea K.\} and Cristian Valencia and Valencia, \{Braulio M.\} and Alejandro Llanos-Cuentas and \{Van Der Auwera\}, Gert and Dujardin, \{Jean Claude\} and Jorge Arevalo",

year = "2012",

month = feb,

doi = "10.1128/JCM.05061-11",

language = "Ingl{\'e}s",

volume = "50",

pages = "495--498",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

number = "2",

}

Veland, N, Boggild, AK, Valencia, C, Valencia, BM, Llanos-Cuentas, A, Van Der Auwera, G, Dujardin, JC & Arevalo, J 2012, 'Leishmania (Viannia) species identification on clinical samples from cutaneous leishmaniasis patients in Peru: Assessment of a molecular stepwise approach', Journal of Clinical Microbiology, vol. 50, no. 2, pp. 495-498. https://doi.org/10.1128/JCM.05061-11

TY - JOUR

T1 - Leishmania (Viannia) species identification on clinical samples from cutaneous leishmaniasis patients in Peru

T2 - Assessment of a molecular stepwise approach

AU - Veland, Nicolas

AU - Boggild, Andrea K.

AU - Valencia, Cristian

AU - Valencia, Braulio M.

AU - Llanos-Cuentas, Alejandro

AU - Van Der Auwera, Gert

AU - Dujardin, Jean Claude

AU - Arevalo, Jorge

PY - 2012/2

Y1 - 2012/2

N2 - Wepresent an algorithm based on three PCR assays for Leishmania (Viannia) species identification and assessed its performance using 70 specimens from Peruvian patients. The succession of the assayed targets can be ordered according to species prevalence. Sequential progression through the algorithm reduced the number of samples here studied by approximately 30% after each step.

AB - Wepresent an algorithm based on three PCR assays for Leishmania (Viannia) species identification and assessed its performance using 70 specimens from Peruvian patients. The succession of the assayed targets can be ordered according to species prevalence. Sequential progression through the algorithm reduced the number of samples here studied by approximately 30% after each step.

UR - https://www.scopus.com/pages/publications/84856162614

U2 - 10.1128/JCM.05061-11

DO - 10.1128/JCM.05061-11

M3 - Artículo

C2 - 22116151

AN - SCOPUS:84856162614

SN - 0095-1137

VL - 50

SP - 495

EP - 498

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 2

ER -

Leishmania (Viannia) species identification on clinical samples from cutaneous leishmaniasis patients in Peru: Assessment of a molecular stepwise approach

Abstract

Access to Document

Other files and links

Fingerprint

Cite this