TY - JOUR
T1 - Molecular typing of IberoAmerican Cryptococcus neoformans isolates
AU - Meyer, Wieland
AU - Castañeda, Alexandra
AU - Jackson, Stuart
AU - Huynh, Matthew
AU - Castañeda, Elizabeth
AU - Arechavala, Alicia
AU - Davel, Graciela
AU - Rodero, Laura
AU - Perrotta, Diego
AU - Lazera, Marcia
AU - Pereira-Igreja, Ricardo
AU - Wanke, Bodo
AU - Mendes-Giannini, Maria Jose
AU - Melhem, Marcia S.C.
AU - Henning-Vainstein, Marlene
AU - Diaz, Maria Cristina
AU - Restrepo, Angela
AU - Huérfano, Sandra
AU - Samayoa, Blanca
AU - Logeman, Heidi
AU - Martirez, Rubén López
AU - Olivares, Laura Rocio Castañon
AU - Contreras-Peres, Cuadberto
AU - Tovar, José Francisco Valenzuela
AU - Bustamante, Beatriz
AU - Torres-Rodriquez, Joseph
AU - Morera, Yolanda
AU - Calvo, Belinda
PY - 2003/2/1
Y1 - 2003/2/1
N2 - A network was established to acquire basic knowledge of Cryptococcus neoformans in IberoAmerican countries. To this effect, 340 clinical, veterinary, and environmental isolates from Argentina, Brazil, Chile, Colombia, Mexico, Peru, Venezuela, Guatemala, and Spain were typed by using M13 polymerase chain reaction-fingerprinting and orotidine monophosphate pyrophosphorylase (URA5) gene restriction fragment length polymorphsm analysis with Hhal and Sau961 in a double digest. Both techniques grouped all isolates into eight previously established molecular types. The majority of the isolates, 68.2% (n=232), were VNI (var. grubii, serotype A), which accords with the fact that this variety causes most human cryptococcal infections worldwide. A smaller proportion, 5.6% (n=19), were VNII (var. grubii, serotype A); 4.1% (n=14), VNIII (AD hybrid), with 9 isolates having a polymorphism in the URA5 gene; 1.8% (n=6), VNIV (var. neoformans, serotype D); 3.5% (n=12), VGI; 6.2% (n=21), VGII; 9.1% (n=31), VGIII, and 1.5% (n=5) VGIV, with all four VG types containing var. gattii serotypes B and C isolates.
AB - A network was established to acquire basic knowledge of Cryptococcus neoformans in IberoAmerican countries. To this effect, 340 clinical, veterinary, and environmental isolates from Argentina, Brazil, Chile, Colombia, Mexico, Peru, Venezuela, Guatemala, and Spain were typed by using M13 polymerase chain reaction-fingerprinting and orotidine monophosphate pyrophosphorylase (URA5) gene restriction fragment length polymorphsm analysis with Hhal and Sau961 in a double digest. Both techniques grouped all isolates into eight previously established molecular types. The majority of the isolates, 68.2% (n=232), were VNI (var. grubii, serotype A), which accords with the fact that this variety causes most human cryptococcal infections worldwide. A smaller proportion, 5.6% (n=19), were VNII (var. grubii, serotype A); 4.1% (n=14), VNIII (AD hybrid), with 9 isolates having a polymorphism in the URA5 gene; 1.8% (n=6), VNIV (var. neoformans, serotype D); 3.5% (n=12), VGI; 6.2% (n=21), VGII; 9.1% (n=31), VGIII, and 1.5% (n=5) VGIV, with all four VG types containing var. gattii serotypes B and C isolates.
UR - https://www.scopus.com/pages/publications/0345074117
U2 - 10.3201/eid0902.020246
DO - 10.3201/eid0902.020246
M3 - Artículo
C2 - 12603989
AN - SCOPUS:0345074117
SN - 1080-6040
VL - 9
SP - 189
EP - 195
JO - Emerging Infectious Diseases
JF - Emerging Infectious Diseases
IS - 2
ER -