Newly developed primers for comprehensive amplification of the rpoB gene and detection of rifampin resistance in Mycobacterium tuberculosis

Leen Rigouts; Oscar Nolasco; Pim De Rijk; Elie Nduwamahoro; Armand Van Deun; Andy Ramsay; Jorge Arevalo; Françoise Portaels

doi:10.1128/JCM.01489-06

Newly developed primers for comprehensive amplification of the rpoB gene and detection of rifampin resistance in Mycobacterium tuberculosis

Leen Rigouts
, Oscar Nolasco
, Pim De Rijk
, Elie Nduwamahoro
, Armand Van Deun
, Andy Ramsay
, Jorge Arevalo
, Françoise Portaels

School of Science and Engineering

Institute of Tropical Medicine
Universidad Peruana Cayetano Heredia, Instituto de Medicina Tropical Alexander von Humboldt
World Health Organization

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

New rpoB gene primers for detecting Rif^r in Mycobacterium tuberculosis complex bacteria achieved 100% specificity and 88% (fresh sputa) and 92% (ethanel-preserved sputa) diagnostic sensitivity and detected up to 4 CFU/sample. Of the 99 Rif^r isolates examined, 97% had mutations within cluster 1,2% at codon 176, and 1% at codon 497.

Original language	English
Pages (from-to)	252-254
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	45
Issue number	1
DOIs	https://doi.org/10.1128/JCM.01489-06
State	Published - Jan 2007

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title = "Newly developed primers for comprehensive amplification of the rpoB gene and detection of rifampin resistance in Mycobacterium tuberculosis",

abstract = "New rpoB gene primers for detecting Rifr in Mycobacterium tuberculosis complex bacteria achieved 100\% specificity and 88\% (fresh sputa) and 92\% (ethanel-preserved sputa) diagnostic sensitivity and detected up to 4 CFU/sample. Of the 99 Rifr isolates examined, 97\% had mutations within cluster 1,2\% at codon 176, and 1\% at codon 497.",

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AU - Rigouts, Leen

AU - Nolasco, Oscar

AU - De Rijk, Pim

AU - Nduwamahoro, Elie

AU - Van Deun, Armand

AU - Ramsay, Andy

AU - Arevalo, Jorge

AU - Portaels, Françoise

PY - 2007/1

Y1 - 2007/1

N2 - New rpoB gene primers for detecting Rifr in Mycobacterium tuberculosis complex bacteria achieved 100% specificity and 88% (fresh sputa) and 92% (ethanel-preserved sputa) diagnostic sensitivity and detected up to 4 CFU/sample. Of the 99 Rifr isolates examined, 97% had mutations within cluster 1,2% at codon 176, and 1% at codon 497.

AB - New rpoB gene primers for detecting Rifr in Mycobacterium tuberculosis complex bacteria achieved 100% specificity and 88% (fresh sputa) and 92% (ethanel-preserved sputa) diagnostic sensitivity and detected up to 4 CFU/sample. Of the 99 Rifr isolates examined, 97% had mutations within cluster 1,2% at codon 176, and 1% at codon 497.

UR - https://www.scopus.com/pages/publications/33846248286

U2 - 10.1128/JCM.01489-06

DO - 10.1128/JCM.01489-06

M3 - Artículo

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AN - SCOPUS:33846248286

SN - 0095-1137

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ER -

Newly developed primers for comprehensive amplification of the rpoB gene and detection of rifampin resistance in Mycobacterium tuberculosis

Abstract

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