TY - JOUR
T1 - Optimization of Plasmodium vivax infection of colonized Amazonian Anopheles darlingi
AU - Andrade, Alice O.
AU - Santos, Najara Akira C.
AU - Bastos, Alessandra S.
AU - Pontual, José Daniel C.
AU - Araújo, Cristiane S.
AU - Lima, Analice S.
AU - Martinez, Leandro N.
AU - Ferreira, Amália S.
AU - Aguiar, Anna Caroline C.
AU - Teles, Carolina B.G.
AU - Guido, Rafael V.C.
AU - Santana, Rosa A.
AU - Lopes, Stefanie C.P.
AU - Medeiros, Jansen F.
AU - Rizopoulos, Zaira
AU - Vinetz, Joseph M.
AU - Campo, Brice
AU - Lacerda, Marcus Vinicius G.
AU - Araújo, Maisa S.
N1 - Publisher Copyright:
© 2023, Springer Nature Limited.
PY - 2023/12
Y1 - 2023/12
N2 - Obtaining Plasmodium vivax sporozoites is essential for in vitro culture of liver stage parasites, not only to understand fundamental aspects of parasite biology, but also for drug and vaccine development. A major impediment to establish high-throughput in vitro P. vivax liver stage assays for drug development is obtaining sufficient numbers of sporozoites. To do so, female anopheline mosquitoes have to be fed on blood from P. vivax-infected patients through an artificial membrane-feeding system, which in turns requires a well-established Anopheles colony. In this study we established conditions to provide a robust supply of P. vivax sporozoites. Adding a combination of serum replacement and antibiotics to the membrane-feeding protocol was found to best improve sporozoite production. A simple centrifugation method appears to be a possible tool for rapidly obtaining purified sporozoites with a minimal loss of yield. However, this method needs to be better defined since sporozoite viability and hepatocyte infection were not evaluated.
AB - Obtaining Plasmodium vivax sporozoites is essential for in vitro culture of liver stage parasites, not only to understand fundamental aspects of parasite biology, but also for drug and vaccine development. A major impediment to establish high-throughput in vitro P. vivax liver stage assays for drug development is obtaining sufficient numbers of sporozoites. To do so, female anopheline mosquitoes have to be fed on blood from P. vivax-infected patients through an artificial membrane-feeding system, which in turns requires a well-established Anopheles colony. In this study we established conditions to provide a robust supply of P. vivax sporozoites. Adding a combination of serum replacement and antibiotics to the membrane-feeding protocol was found to best improve sporozoite production. A simple centrifugation method appears to be a possible tool for rapidly obtaining purified sporozoites with a minimal loss of yield. However, this method needs to be better defined since sporozoite viability and hepatocyte infection were not evaluated.
UR - https://www.scopus.com/pages/publications/85174917985
U2 - 10.1038/s41598-023-44556-y
DO - 10.1038/s41598-023-44556-y
M3 - Artículo
C2 - 37875508
AN - SCOPUS:85174917985
SN - 2045-2322
VL - 13
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 18207
ER -