Putative Leishmania hybrids in the Eastern Andean valley of Huanuco, Peru

Jean Claude Dujardin; Anne Laure Bañuls; Alejandro Llanos-Cuentas; Eugenia Alvarez; Simonne DeDoncker; Diane Jacquet; Dominique Le Ray; Jorge Arevalo; Michel Tibayrenc

doi:10.1016/0001-706X(95)00094-U

Putative Leishmania hybrids in the Eastern Andean valley of Huanuco, Peru

Jean Claude Dujardin
, Anne Laure Bañuls
, Alejandro Llanos-Cuentas
, Eugenia Alvarez
, Simonne DeDoncker
, Diane Jacquet
, Dominique Le Ray
, Jorge Arevalo
, Michel Tibayrenc

Institute of Tropical Medicine
Institut de Recherche Pour le Développement, UMR177 IRD/CIR AD INTERTRYP
Universidad Peruana Cayetano Heredia, Instituto de Medicina Tropical Alexander von Humboldt

Research output: Contribution to journal › Article › peer-review

87 Scopus citations

Abstract

During an outbreak of tegumentary leishmaniasis that developed in the 1990s in the Eastern Andean valley of Huanuco, Peru, the coexistence of Andean (uta) and sylvatic leishmaniases was suspected for ecological and geographical reasons, and sympatric sampling was carried out. Seven human isolates of Leishmania were characterized by multilocus enzyme electrophoresis, random amplification of polymorphic DNA and molecular karyotyping. The three methods identified 3 isolates as L. braziliensis, and 4 isolates as putative hybrids with characters of L. braziliensis and L. peruviana. Data from Huanuco are compared to previous results from other areas endemic for uta. Biological and epidemiological implications are discussed.

Original language	English
Pages (from-to)	293-307
Number of pages	15
Journal	Acta Tropica
Volume	59
Issue number	4
DOIs	https://doi.org/10.1016/0001-706X(95)00094-U
State	Published - Aug 1995

Keywords

Enzyme Electrophoresis
Epidemic
L. peruviana
Leishmania braziliensis
Molecular karyotype
RAPD
Recombination

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10.1016/0001-706X(95)00094-U

Cite this

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title = "Putative Leishmania hybrids in the Eastern Andean valley of Huanuco, Peru",

abstract = "During an outbreak of tegumentary leishmaniasis that developed in the 1990s in the Eastern Andean valley of Huanuco, Peru, the coexistence of Andean (uta) and sylvatic leishmaniases was suspected for ecological and geographical reasons, and sympatric sampling was carried out. Seven human isolates of Leishmania were characterized by multilocus enzyme electrophoresis, random amplification of polymorphic DNA and molecular karyotyping. The three methods identified 3 isolates as L. braziliensis, and 4 isolates as putative hybrids with characters of L. braziliensis and L. peruviana. Data from Huanuco are compared to previous results from other areas endemic for uta. Biological and epidemiological implications are discussed.",

keywords = "Enzyme Electrophoresis, Epidemic, L. peruviana, Leishmania braziliensis, Molecular karyotype, RAPD, Recombination",

author = "Dujardin, \{Jean Claude\} and Ba{\~n}uls, \{Anne Laure\} and Alejandro Llanos-Cuentas and Eugenia Alvarez and Simonne DeDoncker and Diane Jacquet and \{Le Ray\}, Dominique and Jorge Arevalo and Michel Tibayrenc",

year = "1995",

month = aug,

doi = "10.1016/0001-706X(95)00094-U",

language = "Ingl{\'e}s",

volume = "59",

pages = "293--307",

journal = "Acta Tropica",

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TY - JOUR

T1 - Putative Leishmania hybrids in the Eastern Andean valley of Huanuco, Peru

AU - Dujardin, Jean Claude

AU - Bañuls, Anne Laure

AU - Llanos-Cuentas, Alejandro

AU - Alvarez, Eugenia

AU - DeDoncker, Simonne

AU - Jacquet, Diane

AU - Le Ray, Dominique

AU - Arevalo, Jorge

AU - Tibayrenc, Michel

PY - 1995/8

Y1 - 1995/8

N2 - During an outbreak of tegumentary leishmaniasis that developed in the 1990s in the Eastern Andean valley of Huanuco, Peru, the coexistence of Andean (uta) and sylvatic leishmaniases was suspected for ecological and geographical reasons, and sympatric sampling was carried out. Seven human isolates of Leishmania were characterized by multilocus enzyme electrophoresis, random amplification of polymorphic DNA and molecular karyotyping. The three methods identified 3 isolates as L. braziliensis, and 4 isolates as putative hybrids with characters of L. braziliensis and L. peruviana. Data from Huanuco are compared to previous results from other areas endemic for uta. Biological and epidemiological implications are discussed.

AB - During an outbreak of tegumentary leishmaniasis that developed in the 1990s in the Eastern Andean valley of Huanuco, Peru, the coexistence of Andean (uta) and sylvatic leishmaniases was suspected for ecological and geographical reasons, and sympatric sampling was carried out. Seven human isolates of Leishmania were characterized by multilocus enzyme electrophoresis, random amplification of polymorphic DNA and molecular karyotyping. The three methods identified 3 isolates as L. braziliensis, and 4 isolates as putative hybrids with characters of L. braziliensis and L. peruviana. Data from Huanuco are compared to previous results from other areas endemic for uta. Biological and epidemiological implications are discussed.

KW - Enzyme Electrophoresis

KW - Epidemic

KW - L. peruviana

KW - Leishmania braziliensis

KW - Molecular karyotype

KW - RAPD

KW - Recombination

UR - https://www.scopus.com/pages/publications/0029144868

U2 - 10.1016/0001-706X(95)00094-U

DO - 10.1016/0001-706X(95)00094-U

M3 - Artículo

C2 - 8533665

AN - SCOPUS:0029144868

SN - 0001-706X

VL - 59

SP - 293

EP - 307

JO - Acta Tropica

JF - Acta Tropica

IS - 4

ER -

Putative Leishmania hybrids in the Eastern Andean valley of Huanuco, Peru

Abstract

Keywords

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