Secretory immunoglobulin A (sIgA) in saliva versus virulence proteins of enteropathogenic Escherichia coli (EPEC) in ill and colonized children

David Durand; Daniela Alvarez; David Diaz; Erik Mercado; Joaquim Ruiz; Theresa J. Ochoa

doi:10.1016/j.eimc.2019.08.006

Secretory immunoglobulin A (sIgA) in saliva versus virulence proteins of enteropathogenic Escherichia coli (EPEC) in ill and colonized children

David Durand
, Daniela Alvarez
, David Diaz
, Erik Mercado
, Joaquim Ruiz
, Theresa J. Ochoa

Universidad Peruana Cayetano Heredia
Universidad Peruana Cayetano Heredia, Instituto de Medicina Tropical Alexander von Humboldt
Universitat de Barcelona
University of Texas Health Science Center at Houston

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Introduction: We evaluated the presence of sIgA in saliva, versus Escherichia coli secreted proteins (Esp) related to the type III secretion system (T3SS), and its semi-quantitative concentration in children under 2 years-old (no longer breastfed) who were previously colonized or infected with enteropathogenic E. coli (EPEC). Methods: We analyzed the presence of sIgA in 40 children, who previously had positive cultures for EPEC associated (n = 17) or not associated (n = 23) with diarrhea, using the Western Blot technique versus E. coli secreted proteins: EspABCD. A semi-quantitative measurement of the reaction for each protein was made by its density peaks (OD). Results: We found sIgA versus all or some EspABCD proteins in both groups. However, the ill patients had higher concentrations of these antibodies than colonized patients. Discussion: The presence of sIgA in saliva could reflect an intestinal immune response and their levels could be related to a greater exposure and/or bacterial load.

Translated title of the contribution	Inmunoglobulina A secretoria en saliva frente a proteínas de virulencia de Escherichia coli enteropatogénica en niños enfermos y colonizados
Original language	English
Pages (from-to)	279-282
Number of pages	4
Journal	Enfermedades Infecciosas y Microbiologia Clinica
Volume	38
Issue number	6
DOIs	https://doi.org/10.1016/j.eimc.2019.08.006
State	Published - 1 Jun 2020

Keywords

Children
Secretory immunoglobulin A in saliva
Type III secretion system
Virulence proteins

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10.1016/j.eimc.2019.08.006

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title = "Secretory immunoglobulin A (sIgA) in saliva versus virulence proteins of enteropathogenic Escherichia coli (EPEC) in ill and colonized children",

abstract = "Introduction: We evaluated the presence of sIgA in saliva, versus Escherichia coli secreted proteins (Esp) related to the type III secretion system (T3SS), and its semi-quantitative concentration in children under 2 years-old (no longer breastfed) who were previously colonized or infected with enteropathogenic E. coli (EPEC). Methods: We analyzed the presence of sIgA in 40 children, who previously had positive cultures for EPEC associated (n = 17) or not associated (n = 23) with diarrhea, using the Western Blot technique versus E. coli secreted proteins: EspABCD. A semi-quantitative measurement of the reaction for each protein was made by its density peaks (OD). Results: We found sIgA versus all or some EspABCD proteins in both groups. However, the ill patients had higher concentrations of these antibodies than colonized patients. Discussion: The presence of sIgA in saliva could reflect an intestinal immune response and their levels could be related to a greater exposure and/or bacterial load.",

keywords = "Children, Secretory immunoglobulin A in saliva, Type III secretion system, Virulence proteins",

author = "David Durand and Daniela Alvarez and David Diaz and Erik Mercado and Joaquim Ruiz and Ochoa, \{Theresa J.\}",

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T1 - Secretory immunoglobulin A (sIgA) in saliva versus virulence proteins of enteropathogenic Escherichia coli (EPEC) in ill and colonized children

AU - Durand, David

AU - Alvarez, Daniela

AU - Diaz, David

AU - Mercado, Erik

AU - Ruiz, Joaquim

AU - Ochoa, Theresa J.

PY - 2020/6/1

Y1 - 2020/6/1

N2 - Introduction: We evaluated the presence of sIgA in saliva, versus Escherichia coli secreted proteins (Esp) related to the type III secretion system (T3SS), and its semi-quantitative concentration in children under 2 years-old (no longer breastfed) who were previously colonized or infected with enteropathogenic E. coli (EPEC). Methods: We analyzed the presence of sIgA in 40 children, who previously had positive cultures for EPEC associated (n = 17) or not associated (n = 23) with diarrhea, using the Western Blot technique versus E. coli secreted proteins: EspABCD. A semi-quantitative measurement of the reaction for each protein was made by its density peaks (OD). Results: We found sIgA versus all or some EspABCD proteins in both groups. However, the ill patients had higher concentrations of these antibodies than colonized patients. Discussion: The presence of sIgA in saliva could reflect an intestinal immune response and their levels could be related to a greater exposure and/or bacterial load.

AB - Introduction: We evaluated the presence of sIgA in saliva, versus Escherichia coli secreted proteins (Esp) related to the type III secretion system (T3SS), and its semi-quantitative concentration in children under 2 years-old (no longer breastfed) who were previously colonized or infected with enteropathogenic E. coli (EPEC). Methods: We analyzed the presence of sIgA in 40 children, who previously had positive cultures for EPEC associated (n = 17) or not associated (n = 23) with diarrhea, using the Western Blot technique versus E. coli secreted proteins: EspABCD. A semi-quantitative measurement of the reaction for each protein was made by its density peaks (OD). Results: We found sIgA versus all or some EspABCD proteins in both groups. However, the ill patients had higher concentrations of these antibodies than colonized patients. Discussion: The presence of sIgA in saliva could reflect an intestinal immune response and their levels could be related to a greater exposure and/or bacterial load.

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KW - Type III secretion system

KW - Virulence proteins

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DO - 10.1016/j.eimc.2019.08.006

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AN - SCOPUS:85074434758

SN - 0213-005X

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JO - Enfermedades Infecciosas y Microbiologia Clinica

JF - Enfermedades Infecciosas y Microbiologia Clinica

IS - 6

ER -

Secretory immunoglobulin A (sIgA) in saliva versus virulence proteins of enteropathogenic Escherichia coli (EPEC) in ill and colonized children

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