Short report: Polymerase chain reaction for chronic Trypanosoma cruzi infection yields higher sensitivity in blood clot than buffy coat or whole blood specimens

Sean Fitzwater; Maritza Calderon; Carlos LaFuente; Gerson Galdos-Cardenas; Lisbeth Ferrufino; Manuela Verastegui; Robert H. Gilman; Caryn Bern

doi:10.4269/ajtmh.2008.79.768

Short report: Polymerase chain reaction for chronic Trypanosoma cruzi infection yields higher sensitivity in blood clot than buffy coat or whole blood specimens

Sean Fitzwater
, Maritza Calderon
, Carlos LaFuente
, Gerson Galdos-Cardenas
, Lisbeth Ferrufino
, Manuela Verastegui
, Robert H. Gilman
, Caryn Bern

Johns Hopkins Bloomberg School of Public Health
Hospital Universitario Japones
Centers for Disease Control and Prevention
Centers for Disease Control and Prevention

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

Trypanosoma cruzi polymerase chain reaction (PCR) is widely used, but sensitivity varies widely. We compared PCR using 121/122 primers targeting kinetoplast minicircle DNA in whole blood, buffy coat, and clot from Bolivian women. Sensitivity was significantly higher in clot (60.1%) than buffy coat (46.5%) or whole blood (40%). The use of clot could simplify specimen collection while improving sensitivity.

Original language	English
Pages (from-to)	768-770
Number of pages	3
Journal	American Journal of Tropical Medicine and Hygiene
Volume	79
Issue number	5
DOIs	https://doi.org/10.4269/ajtmh.2008.79.768
State	Published - Nov 2008

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10.4269/ajtmh.2008.79.768

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Fitzwater, S., Calderon, M., LaFuente, C., Galdos-Cardenas, G., Ferrufino, L., Verastegui, M., Gilman, R. H., & Bern, C. (2008). Short report: Polymerase chain reaction for chronic Trypanosoma cruzi infection yields higher sensitivity in blood clot than buffy coat or whole blood specimens. American Journal of Tropical Medicine and Hygiene, 79(5), 768-770. https://doi.org/10.4269/ajtmh.2008.79.768

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title = "Short report: Polymerase chain reaction for chronic Trypanosoma cruzi infection yields higher sensitivity in blood clot than buffy coat or whole blood specimens",

abstract = "Trypanosoma cruzi polymerase chain reaction (PCR) is widely used, but sensitivity varies widely. We compared PCR using 121/122 primers targeting kinetoplast minicircle DNA in whole blood, buffy coat, and clot from Bolivian women. Sensitivity was significantly higher in clot (60.1\%) than buffy coat (46.5\%) or whole blood (40\%). The use of clot could simplify specimen collection while improving sensitivity.",

author = "Sean Fitzwater and Maritza Calderon and Carlos LaFuente and Gerson Galdos-Cardenas and Lisbeth Ferrufino and Manuela Verastegui and Gilman, \{Robert H.\} and Caryn Bern",

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Fitzwater, S, Calderon, M, LaFuente, C, Galdos-Cardenas, G, Ferrufino, L, Verastegui, M, Gilman, RH & Bern, C 2008, 'Short report: Polymerase chain reaction for chronic Trypanosoma cruzi infection yields higher sensitivity in blood clot than buffy coat or whole blood specimens', American Journal of Tropical Medicine and Hygiene, vol. 79, no. 5, pp. 768-770. https://doi.org/10.4269/ajtmh.2008.79.768

Short report: Polymerase chain reaction for chronic Trypanosoma cruzi infection yields higher sensitivity in blood clot than buffy coat or whole blood specimens. / Fitzwater, Sean; Calderon, Maritza; LaFuente, Carlos et al.
In: American Journal of Tropical Medicine and Hygiene, Vol. 79, No. 5, 11.2008, p. 768-770.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Short report

T2 - Polymerase chain reaction for chronic Trypanosoma cruzi infection yields higher sensitivity in blood clot than buffy coat or whole blood specimens

AU - Fitzwater, Sean

AU - Calderon, Maritza

AU - LaFuente, Carlos

AU - Galdos-Cardenas, Gerson

AU - Ferrufino, Lisbeth

AU - Verastegui, Manuela

AU - Gilman, Robert H.

AU - Bern, Caryn

PY - 2008/11

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N2 - Trypanosoma cruzi polymerase chain reaction (PCR) is widely used, but sensitivity varies widely. We compared PCR using 121/122 primers targeting kinetoplast minicircle DNA in whole blood, buffy coat, and clot from Bolivian women. Sensitivity was significantly higher in clot (60.1%) than buffy coat (46.5%) or whole blood (40%). The use of clot could simplify specimen collection while improving sensitivity.

AB - Trypanosoma cruzi polymerase chain reaction (PCR) is widely used, but sensitivity varies widely. We compared PCR using 121/122 primers targeting kinetoplast minicircle DNA in whole blood, buffy coat, and clot from Bolivian women. Sensitivity was significantly higher in clot (60.1%) than buffy coat (46.5%) or whole blood (40%). The use of clot could simplify specimen collection while improving sensitivity.

UR - https://www.scopus.com/pages/publications/55849112284

U2 - 10.4269/ajtmh.2008.79.768

DO - 10.4269/ajtmh.2008.79.768

M3 - Artículo

C2 - 18981520

AN - SCOPUS:55849112284

SN - 0002-9637

VL - 79

SP - 768

EP - 770

JO - American Journal of Tropical Medicine and Hygiene

JF - American Journal of Tropical Medicine and Hygiene

IS - 5

ER -

Short report: Polymerase chain reaction for chronic Trypanosoma cruzi infection yields higher sensitivity in blood clot than buffy coat or whole blood specimens

Abstract

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