The use of nonradioactive DNA probes for the characterization of Leishmania isolates from Peru

M. Lopez; Y. Montoya; M. Arana; F. Cruzalegui; J. Braga; A. Llanos-Cuentas; G. Romero; J. Arevalo

doi:10.4269/ajtmh.1988.38.308

The use of nonradioactive DNA probes for the characterization of Leishmania isolates from Peru

M. Lopez
, Y. Montoya
, M. Arana
, F. Cruzalegui
, J. Braga
, A. Llanos-Cuentas
, G. Romero
, J. Arevalo

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

We describe conditions for dot blot DNA hybridization studies using biotinylated kDNA probes from Leishmania. The sensitivity and specificity attained with biotinylated or ³²P-labeled probes were equivalent. The lower level of detection obtained was 100 parasites that were blotted on nitrocellulose paper and then treated with Proteinase K. Studies were performed with 112 Leishmania isolates from Andean (uta) and sylvatic mucocutaneous (espundia) patients and all were determined to belong to the Leishmania braziliensis complex.

Original language	English
Pages (from-to)	308-314
Number of pages	7
Journal	American Journal of Tropical Medicine and Hygiene
Volume	38
Issue number	2
DOIs	https://doi.org/10.4269/ajtmh.1988.38.308
State	Published - 1988

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10.4269/ajtmh.1988.38.308

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title = "The use of nonradioactive DNA probes for the characterization of Leishmania isolates from Peru",

abstract = "We describe conditions for dot blot DNA hybridization studies using biotinylated kDNA probes from Leishmania. The sensitivity and specificity attained with biotinylated or 32P-labeled probes were equivalent. The lower level of detection obtained was 100 parasites that were blotted on nitrocellulose paper and then treated with Proteinase K. Studies were performed with 112 Leishmania isolates from Andean (uta) and sylvatic mucocutaneous (espundia) patients and all were determined to belong to the Leishmania braziliensis complex.",

author = "M. Lopez and Y. Montoya and M. Arana and F. Cruzalegui and J. Braga and A. Llanos-Cuentas and G. Romero and J. Arevalo",

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T1 - The use of nonradioactive DNA probes for the characterization of Leishmania isolates from Peru

AU - Lopez, M.

AU - Montoya, Y.

AU - Arana, M.

AU - Cruzalegui, F.

AU - Braga, J.

AU - Llanos-Cuentas, A.

AU - Romero, G.

AU - Arevalo, J.

PY - 1988

Y1 - 1988

N2 - We describe conditions for dot blot DNA hybridization studies using biotinylated kDNA probes from Leishmania. The sensitivity and specificity attained with biotinylated or 32P-labeled probes were equivalent. The lower level of detection obtained was 100 parasites that were blotted on nitrocellulose paper and then treated with Proteinase K. Studies were performed with 112 Leishmania isolates from Andean (uta) and sylvatic mucocutaneous (espundia) patients and all were determined to belong to the Leishmania braziliensis complex.

AB - We describe conditions for dot blot DNA hybridization studies using biotinylated kDNA probes from Leishmania. The sensitivity and specificity attained with biotinylated or 32P-labeled probes were equivalent. The lower level of detection obtained was 100 parasites that were blotted on nitrocellulose paper and then treated with Proteinase K. Studies were performed with 112 Leishmania isolates from Andean (uta) and sylvatic mucocutaneous (espundia) patients and all were determined to belong to the Leishmania braziliensis complex.

UR - https://www.scopus.com/pages/publications/0023846220

U2 - 10.4269/ajtmh.1988.38.308

DO - 10.4269/ajtmh.1988.38.308

M3 - Artículo

C2 - 3354765

AN - SCOPUS:0023846220

SN - 0002-9637

VL - 38

SP - 308

EP - 314

JO - American Journal of Tropical Medicine and Hygiene

JF - American Journal of Tropical Medicine and Hygiene

IS - 2

ER -

The use of nonradioactive DNA probes for the characterization of Leishmania isolates from Peru

Abstract

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