Use of a reliable PCR assay for the detection of Neisseria gonorrhoeae in Peruvian patients

H. Mayta; M. Calderon; J. Taverna; S. Montenegro; J. Balqui; K. Campos; I. Tuero; J. Arevalo; A. Vivar; R. H. Gilman

doi:10.1111/j.1469-0691.2006.01452.x

Use of a reliable PCR assay for the detection of Neisseria gonorrhoeae in Peruvian patients

H. Mayta
, M. Calderon
, J. Taverna
, S. Montenegro
, J. Balqui
, K. Campos
, I. Tuero
, J. Arevalo
, A. Vivar
, R. H. Gilman

School of Science and Engineering

Boston University Medical Center
Ochsner Clinic Foundation
Universidad Peruana Cayetano Heredia
Hospital Nacional Dos de Mayo
Arzobispo Loayza Hospital
Johns Hopkins Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Neisseria gonorrhoeae is the most common sexually transmitted disease-causing bacterium worldwide. An in-house PCR assay targeting the carbamoyl-phosphate synthase subunit A (carA) gene was developed for the specific detection of N. gonorrhoeae in clinical specimens. Samples from 605 patients were cultured on selective medium and assayed by PCR in a double-blind fashion. Of 605 urethral/cervical samples analysed, 13 were PCR-positive, of which 11 were culture-positive. The PCR showed a sensitivity and specificity of 100% and 99.7% with these samples. PCR targeting the carA gene appears to be a reliable method for the detection of N. gonorrhoeae in clinical specimens.

Original language	English
Pages (from-to)	809-812
Number of pages	4
Journal	Clinical Microbiology and Infection
Volume	12
Issue number	8
DOIs	https://doi.org/10.1111/j.1469-0691.2006.01452.x
State	Published - Aug 2006

Keywords

Diagnosis
Neisseria gonorrhoeae
PCR
Sexually transmitted disease
Swab samples
carA gene

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10.1111/j.1469-0691.2006.01452.x

Cite this

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title = "Use of a reliable PCR assay for the detection of Neisseria gonorrhoeae in Peruvian patients",

abstract = "Neisseria gonorrhoeae is the most common sexually transmitted disease-causing bacterium worldwide. An in-house PCR assay targeting the carbamoyl-phosphate synthase subunit A (carA) gene was developed for the specific detection of N. gonorrhoeae in clinical specimens. Samples from 605 patients were cultured on selective medium and assayed by PCR in a double-blind fashion. Of 605 urethral/cervical samples analysed, 13 were PCR-positive, of which 11 were culture-positive. The PCR showed a sensitivity and specificity of 100\% and 99.7\% with these samples. PCR targeting the carA gene appears to be a reliable method for the detection of N. gonorrhoeae in clinical specimens.",

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T1 - Use of a reliable PCR assay for the detection of Neisseria gonorrhoeae in Peruvian patients

AU - Mayta, H.

AU - Calderon, M.

AU - Taverna, J.

AU - Montenegro, S.

AU - Balqui, J.

AU - Campos, K.

AU - Tuero, I.

AU - Arevalo, J.

AU - Vivar, A.

AU - Gilman, R. H.

PY - 2006/8

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N2 - Neisseria gonorrhoeae is the most common sexually transmitted disease-causing bacterium worldwide. An in-house PCR assay targeting the carbamoyl-phosphate synthase subunit A (carA) gene was developed for the specific detection of N. gonorrhoeae in clinical specimens. Samples from 605 patients were cultured on selective medium and assayed by PCR in a double-blind fashion. Of 605 urethral/cervical samples analysed, 13 were PCR-positive, of which 11 were culture-positive. The PCR showed a sensitivity and specificity of 100% and 99.7% with these samples. PCR targeting the carA gene appears to be a reliable method for the detection of N. gonorrhoeae in clinical specimens.

AB - Neisseria gonorrhoeae is the most common sexually transmitted disease-causing bacterium worldwide. An in-house PCR assay targeting the carbamoyl-phosphate synthase subunit A (carA) gene was developed for the specific detection of N. gonorrhoeae in clinical specimens. Samples from 605 patients were cultured on selective medium and assayed by PCR in a double-blind fashion. Of 605 urethral/cervical samples analysed, 13 were PCR-positive, of which 11 were culture-positive. The PCR showed a sensitivity and specificity of 100% and 99.7% with these samples. PCR targeting the carA gene appears to be a reliable method for the detection of N. gonorrhoeae in clinical specimens.

KW - Diagnosis

KW - Neisseria gonorrhoeae

KW - PCR

KW - Sexually transmitted disease

KW - Swab samples

KW - carA gene

UR - https://www.scopus.com/pages/publications/33745590844

U2 - 10.1111/j.1469-0691.2006.01452.x

DO - 10.1111/j.1469-0691.2006.01452.x

M3 - Artículo

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AN - SCOPUS:33745590844

SN - 1198-743X

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JO - Clinical Microbiology and Infection

JF - Clinical Microbiology and Infection

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ER -

Use of a reliable PCR assay for the detection of Neisseria gonorrhoeae in Peruvian patients

Abstract

Keywords

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