Validation of five-colony pool analysis using multiplex real-time PCR for detection of diarrheagenic Escherichia coli

F. Barletta; T. J. Ochoa; L. Ecker; A. I. Gil; C. F. Lanata; T. G. Cleary

doi:10.1128/JCM.00608-09

Validation of five-colony pool analysis using multiplex real-time PCR for detection of diarrheagenic Escherichia coli

F. Barletta
, T. J. Ochoa
, L. Ecker
, A. I. Gil
, C. F. Lanata
, T. G. Cleary

School of Medicine

Universidad Peruana Cayetano Heredia
University of Texas Health Science Center at Houston
Instituto de Investigación Nutricional

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

Five Escherichia coli colonies/patient were studied to evaluate the reliability of a multiplex real-time PCR assay for detection of diarrheagenic Escherichia coli groups, using a pool of five colonies rather than individual colonies. Sensitivity and specificity were 98% and 100%, respectively, at a fifth of the cost of the individual colony analysis.

Original language	English
Pages (from-to)	1915-1917
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	47
Issue number	6
DOIs	https://doi.org/10.1128/JCM.00608-09
State	Published - Jun 2009

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title = "Validation of five-colony pool analysis using multiplex real-time PCR for detection of diarrheagenic Escherichia coli",

abstract = "Five Escherichia coli colonies/patient were studied to evaluate the reliability of a multiplex real-time PCR assay for detection of diarrheagenic Escherichia coli groups, using a pool of five colonies rather than individual colonies. Sensitivity and specificity were 98\% and 100\%, respectively, at a fifth of the cost of the individual colony analysis.",

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AU - Barletta, F.

AU - Ochoa, T. J.

AU - Ecker, L.

AU - Gil, A. I.

AU - Lanata, C. F.

AU - Cleary, T. G.

PY - 2009/6

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AB - Five Escherichia coli colonies/patient were studied to evaluate the reliability of a multiplex real-time PCR assay for detection of diarrheagenic Escherichia coli groups, using a pool of five colonies rather than individual colonies. Sensitivity and specificity were 98% and 100%, respectively, at a fifth of the cost of the individual colony analysis.

UR - https://www.scopus.com/pages/publications/66749151049

U2 - 10.1128/JCM.00608-09

DO - 10.1128/JCM.00608-09

M3 - Artículo

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AN - SCOPUS:66749151049

SN - 0095-1137

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EP - 1917

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 6

ER -

Validation of five-colony pool analysis using multiplex real-time PCR for detection of diarrheagenic Escherichia coli

Abstract

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