CRISPR-Cas13d Induces Efficient mRNA Knockdown in Animal Embryos

Gopal Kushawah; Luis Hernandez-Huertas; Joaquin Abugattas-Nuñez del Prado; Juan R. Martinez-Morales; Michelle L. DeVore; Huzaifa Hassan; Ismael Moreno-Sanchez; Laura Tomas-Gallardo; Alejandro Diaz-Moscoso; Dahiana E. Monges; Javier R. Guelfo; William C. Theune; Emry O. Brannan; Wei Wang; Timothy J. Corbin; Andrea M. Moran; Alejandro Sánchez Alvarado; Edward Málaga-Trillo; Carter M. Takacs; Ariel A. Bazzini; Miguel A. Moreno-Mateos

doi:10.1016/j.devcel.2020.07.013

CRISPR-Cas13d Induces Efficient mRNA Knockdown in Animal Embryos

Gopal Kushawah
, Luis Hernandez-Huertas
, Joaquin Abugattas-Nuñez del Prado
, Juan R. Martinez-Morales
, Michelle L. DeVore
, Huzaifa Hassan
, Ismael Moreno-Sanchez
, Laura Tomas-Gallardo
, Alejandro Diaz-Moscoso
, Dahiana E. Monges
, Javier R. Guelfo
, William C. Theune
, Emry O. Brannan
, Wei Wang
, Timothy J. Corbin
, Andrea M. Moran
, Alejandro Sánchez Alvarado
, Edward Málaga-Trillo
, Carter M. Takacs
, Ariel A. Bazzini

Miguel A. Moreno-Mateos

Stowers Institute for Medical Research
Universidad Pablo de Olavide, de Sevilla
Universidad Peruana Cayetano Heredia
Andalusian Center for Developmental Biology/Pablo de Olavide University/CSIC/Junta de Andalucía
University of New Haven
Boston Children's Hospital and the Howard Hughes Medical Institute
University of Kansas Medical Center

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

179 Citas (Scopus)

Resumen

The development of mRNA knockdown technologies for use in vertebrate organisms such as zebrafish has been limited. Kushawah et al. establish CRISPR-RfxCas13d as an efficient, specific, cost-effective, and straightforward method for the systematic and tractable study of gene function in vivo during embryogenesis across a range of animal species.

Idioma original	Inglés
Páginas (desde-hasta)	805-817.e7
Publicación	Developmental Cell
Volumen	54
N.º	6
DOI	https://doi.org/10.1016/j.devcel.2020.07.013
Estado	Publicada - 28 set. 2020

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Kushawah, G., Hernandez-Huertas, L., Abugattas-Nuñez del Prado, J., Martinez-Morales, J. R., DeVore, M. L., Hassan, H., Moreno-Sanchez, I., Tomas-Gallardo, L., Diaz-Moscoso, A., Monges, D. E., Guelfo, J. R., Theune, W. C., Brannan, E. O., Wang, W., Corbin, T. J., Moran, A. M., Sánchez Alvarado, A., Málaga-Trillo, E., Takacs, C. M., ... Moreno-Mateos, M. A. (2020). CRISPR-Cas13d Induces Efficient mRNA Knockdown in Animal Embryos. Developmental Cell, 54(6), 805-817.e7. https://doi.org/10.1016/j.devcel.2020.07.013

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title = "CRISPR-Cas13d Induces Efficient mRNA Knockdown in Animal Embryos",

abstract = "The development of mRNA knockdown technologies for use in vertebrate organisms such as zebrafish has been limited. Kushawah et al. establish CRISPR-RfxCas13d as an efficient, specific, cost-effective, and straightforward method for the systematic and tractable study of gene function in vivo during embryogenesis across a range of animal species.",

keywords = "CRISPR-Cas13, Cas13d, MZT, RNA targeting, early development, embryogenesis, killifish, knockdown, medaka, zebrafish",

author = "Gopal Kushawah and Luis Hernandez-Huertas and \{Abugattas-Nu{\~n}ez del Prado\}, Joaquin and Martinez-Morales, \{Juan R.\} and DeVore, \{Michelle L.\} and Huzaifa Hassan and Ismael Moreno-Sanchez and Laura Tomas-Gallardo and Alejandro Diaz-Moscoso and Monges, \{Dahiana E.\} and Guelfo, \{Javier R.\} and Theune, \{William C.\} and Brannan, \{Emry O.\} and Wei Wang and Corbin, \{Timothy J.\} and Moran, \{Andrea M.\} and \{S{\'a}nchez Alvarado\}, Alejandro and Edward M{\'a}laga-Trillo and Takacs, \{Carter M.\} and Bazzini, \{Ariel A.\} and Moreno-Mateos, \{Miguel A.\}",

note = "Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",

year = "2020",

month = sep,

day = "28",

doi = "10.1016/j.devcel.2020.07.013",

language = "Ingl{\'e}s",

volume = "54",

pages = "805--817.e7",

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Kushawah, G, Hernandez-Huertas, L, Abugattas-Nuñez del Prado, J, Martinez-Morales, JR, DeVore, ML, Hassan, H, Moreno-Sanchez, I, Tomas-Gallardo, L, Diaz-Moscoso, A, Monges, DE, Guelfo, JR, Theune, WC, Brannan, EO, Wang, W, Corbin, TJ, Moran, AM, Sánchez Alvarado, A, Málaga-Trillo, E, Takacs, CM, Bazzini, AA & Moreno-Mateos, MA 2020, 'CRISPR-Cas13d Induces Efficient mRNA Knockdown in Animal Embryos', Developmental Cell, vol. 54, n.º 6, pp. 805-817.e7. https://doi.org/10.1016/j.devcel.2020.07.013

TY - JOUR

T1 - CRISPR-Cas13d Induces Efficient mRNA Knockdown in Animal Embryos

AU - Kushawah, Gopal

AU - Hernandez-Huertas, Luis

AU - Abugattas-Nuñez del Prado, Joaquin

AU - Martinez-Morales, Juan R.

AU - DeVore, Michelle L.

AU - Hassan, Huzaifa

AU - Moreno-Sanchez, Ismael

AU - Tomas-Gallardo, Laura

AU - Diaz-Moscoso, Alejandro

AU - Monges, Dahiana E.

AU - Guelfo, Javier R.

AU - Theune, William C.

AU - Brannan, Emry O.

AU - Wang, Wei

AU - Corbin, Timothy J.

AU - Moran, Andrea M.

AU - Sánchez Alvarado, Alejandro

AU - Málaga-Trillo, Edward

AU - Takacs, Carter M.

AU - Bazzini, Ariel A.

AU - Moreno-Mateos, Miguel A.

PY - 2020/9/28

Y1 - 2020/9/28

N2 - The development of mRNA knockdown technologies for use in vertebrate organisms such as zebrafish has been limited. Kushawah et al. establish CRISPR-RfxCas13d as an efficient, specific, cost-effective, and straightforward method for the systematic and tractable study of gene function in vivo during embryogenesis across a range of animal species.

AB - The development of mRNA knockdown technologies for use in vertebrate organisms such as zebrafish has been limited. Kushawah et al. establish CRISPR-RfxCas13d as an efficient, specific, cost-effective, and straightforward method for the systematic and tractable study of gene function in vivo during embryogenesis across a range of animal species.

KW - CRISPR-Cas13

KW - Cas13d

KW - MZT

KW - RNA targeting

KW - early development

KW - embryogenesis

KW - killifish

KW - knockdown

KW - medaka

KW - zebrafish

UR - https://www.scopus.com/pages/publications/85089988712

U2 - 10.1016/j.devcel.2020.07.013

DO - 10.1016/j.devcel.2020.07.013

M3 - Artículo

C2 - 32768421

AN - SCOPUS:85089988712

SN - 1534-5807

VL - 54

SP - 805-817.e7

JO - Developmental Cell

JF - Developmental Cell

IS - 6

ER -

CRISPR-Cas13d Induces Efficient mRNA Knockdown in Animal Embryos

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