Detection of Mycobacterium Tuberculosis DNA in Buccal Swab Samples from Children in Lima, Peru

Juan A. Flores; Roger Calderón; Annelies W. Mesman; Martin Soto; Julia Coit; Juan Aliaga; Milagros Mendoza; Segundo R. Leon; Kelika Konda; Francisco M. Mestanza; Carlos J. Mendoza; Leonid Lecca; Megan B. Murray; Rebecca C. Holmberg; Nira R. Pollock; Molly F. Franke

doi:10.1097/INF.0000000000002828

Detection of Mycobacterium Tuberculosis DNA in Buccal Swab Samples from Children in Lima, Peru

Juan A. Flores
, Roger Calderón
, Annelies W. Mesman
, Martin Soto
, Julia Coit
, Juan Aliaga
, Milagros Mendoza
, Segundo R. Leon
, Kelika Konda
, Francisco M. Mestanza
, Carlos J. Mendoza
, Leonid Lecca
, Megan B. Murray
, Rebecca C. Holmberg
, Nira R. Pollock
, Molly F. Franke

Centro de Investigación Interdisciplinaria en Sexualidad, Sida y Sociedad

San Juan Bautista Private University
Universidad Peruana Cayetano Heredia
Socios en Salud Lima
Universidade Federal do Rio de Janeiro
Harvard Medical School
San Bartolomé Mother-Child National Teaching Hospital
Ministry of Health of Peru
Akonni Biosystems Inc
Dana-Farber Cancer Institute

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26 Citas (Scopus)

Resumen

We examined Mycobacterium tuberculosis DNA detection from buccal swab samples collected from children in Lima, Peru. DNA was extracted and amplified via real-time polymerase chain reaction. Sensitivity was 21% (95% confidence interval [CI]: 7%-42%) in 24 culture-confirmed tuberculosis cases and 4.6% (95% CI: 1%-13%) in 65 clinically diagnosed unconfirmed cases. Sensitivity was highest for smear-positive tuberculosis. Specificity was 99% in the 199 controls (95% CI: 96%-100%).

Idioma original	Inglés
Páginas (desde-hasta)	E376-E380
Publicación	Pediatric Infectious Disease Journal
Volumen	39
N.º	11
DOI	https://doi.org/10.1097/INF.0000000000002828
Estado	Publicada - 1 nov. 2020

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Flores, J. A., Calderón, R., Mesman, A. W., Soto, M., Coit, J., Aliaga, J., Mendoza, M., Leon, S. R., Konda, K., Mestanza, F. M., Mendoza, C. J., Lecca, L., Murray, M. B., Holmberg, R. C., Pollock, N. R., & Franke, M. F. (2020). Detection of Mycobacterium Tuberculosis DNA in Buccal Swab Samples from Children in Lima, Peru. Pediatric Infectious Disease Journal, 39(11), E376-E380. https://doi.org/10.1097/INF.0000000000002828

@article{26e564298d9b44d687aef3a01c48db97,

title = "Detection of Mycobacterium Tuberculosis DNA in Buccal Swab Samples from Children in Lima, Peru",

abstract = "We examined Mycobacterium tuberculosis DNA detection from buccal swab samples collected from children in Lima, Peru. DNA was extracted and amplified via real-time polymerase chain reaction. Sensitivity was 21\% (95\% confidence interval [CI]: 7\%-42\%) in 24 culture-confirmed tuberculosis cases and 4.6\% (95\% CI: 1\%-13\%) in 65 clinically diagnosed unconfirmed cases. Sensitivity was highest for smear-positive tuberculosis. Specificity was 99\% in the 199 controls (95\% CI: 96\%-100\%).",

keywords = "childhood tuberculosis, diagnostic, oral swab, real-time polymerase chain reaction, tuberculosis",

author = "Flores, \{Juan A.\} and Roger Calder{\'o}n and Mesman, \{Annelies W.\} and Martin Soto and Julia Coit and Juan Aliaga and Milagros Mendoza and Leon, \{Segundo R.\} and Kelika Konda and Mestanza, \{Francisco M.\} and Mendoza, \{Carlos J.\} and Leonid Lecca and Murray, \{Megan B.\} and Holmberg, \{Rebecca C.\} and Pollock, \{Nira R.\} and Franke, \{Molly F.\}",

year = "2020",

month = nov,

day = "1",

doi = "10.1097/INF.0000000000002828",

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Flores, JA, Calderón, R, Mesman, AW, Soto, M, Coit, J, Aliaga, J, Mendoza, M, Leon, SR, Konda, K, Mestanza, FM, Mendoza, CJ, Lecca, L, Murray, MB, Holmberg, RC, Pollock, NR & Franke, MF 2020, 'Detection of Mycobacterium Tuberculosis DNA in Buccal Swab Samples from Children in Lima, Peru', Pediatric Infectious Disease Journal, vol. 39, n.º 11, pp. E376-E380. https://doi.org/10.1097/INF.0000000000002828

TY - JOUR

T1 - Detection of Mycobacterium Tuberculosis DNA in Buccal Swab Samples from Children in Lima, Peru

AU - Flores, Juan A.

AU - Calderón, Roger

AU - Mesman, Annelies W.

AU - Soto, Martin

AU - Coit, Julia

AU - Aliaga, Juan

AU - Mendoza, Milagros

AU - Leon, Segundo R.

AU - Konda, Kelika

AU - Mestanza, Francisco M.

AU - Mendoza, Carlos J.

AU - Lecca, Leonid

AU - Murray, Megan B.

AU - Holmberg, Rebecca C.

AU - Pollock, Nira R.

AU - Franke, Molly F.

PY - 2020/11/1

Y1 - 2020/11/1

N2 - We examined Mycobacterium tuberculosis DNA detection from buccal swab samples collected from children in Lima, Peru. DNA was extracted and amplified via real-time polymerase chain reaction. Sensitivity was 21% (95% confidence interval [CI]: 7%-42%) in 24 culture-confirmed tuberculosis cases and 4.6% (95% CI: 1%-13%) in 65 clinically diagnosed unconfirmed cases. Sensitivity was highest for smear-positive tuberculosis. Specificity was 99% in the 199 controls (95% CI: 96%-100%).

AB - We examined Mycobacterium tuberculosis DNA detection from buccal swab samples collected from children in Lima, Peru. DNA was extracted and amplified via real-time polymerase chain reaction. Sensitivity was 21% (95% confidence interval [CI]: 7%-42%) in 24 culture-confirmed tuberculosis cases and 4.6% (95% CI: 1%-13%) in 65 clinically diagnosed unconfirmed cases. Sensitivity was highest for smear-positive tuberculosis. Specificity was 99% in the 199 controls (95% CI: 96%-100%).

KW - childhood tuberculosis

KW - diagnostic

KW - oral swab

KW - real-time polymerase chain reaction

KW - tuberculosis

UR - https://www.scopus.com/pages/publications/85093908062

U2 - 10.1097/INF.0000000000002828

DO - 10.1097/INF.0000000000002828

M3 - Artículo

C2 - 32675773

AN - SCOPUS:85093908062

SN - 0891-3668

VL - 39

SP - E376-E380

JO - Pediatric Infectious Disease Journal

JF - Pediatric Infectious Disease Journal

IS - 11

ER -

Detection of Mycobacterium Tuberculosis DNA in Buccal Swab Samples from Children in Lima, Peru

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