Leishmania (Viannia) species identification on clinical samples from cutaneous leishmaniasis patients in Peru: Assessment of a molecular stepwise approach

Nicolas Veland; Andrea K. Boggild; Cristian Valencia; Braulio M. Valencia; Alejandro Llanos-Cuentas; Gert Van Der Auwera; Jean Claude Dujardin; Jorge Arevalo

doi:10.1128/JCM.05061-11

Leishmania (Viannia) species identification on clinical samples from cutaneous leishmaniasis patients in Peru: Assessment of a molecular stepwise approach

Nicolas Veland
, Andrea K. Boggild
, Cristian Valencia
, Braulio M. Valencia
, Alejandro Llanos-Cuentas
, Gert Van Der Auwera
, Jean Claude Dujardin
, Jorge Arevalo

Universidad Peruana Cayetano Heredia, Instituto de Medicina Tropical Alexander von Humboldt
Toronto General Hospital
Hospital Nacional Cayetano Heredia
Institute of Tropical Medicine
University of Antwerp

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30 Citas (Scopus)

Resumen

Wepresent an algorithm based on three PCR assays for Leishmania (Viannia) species identification and assessed its performance using 70 specimens from Peruvian patients. The succession of the assayed targets can be ordered according to species prevalence. Sequential progression through the algorithm reduced the number of samples here studied by approximately 30% after each step.

Idioma original	Inglés
Páginas (desde-hasta)	495-498
Número de páginas	4
Publicación	Journal of Clinical Microbiology
Volumen	50
N.º	2
DOI	https://doi.org/10.1128/JCM.05061-11
Estado	Publicada - feb. 2012

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10.1128/JCM.05061-11

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Veland, N., Boggild, A. K., Valencia, C., Valencia, B. M., Llanos-Cuentas, A., Van Der Auwera, G., Dujardin, J. C., & Arevalo, J. (2012). Leishmania (Viannia) species identification on clinical samples from cutaneous leishmaniasis patients in Peru: Assessment of a molecular stepwise approach. Journal of Clinical Microbiology, 50(2), 495-498. https://doi.org/10.1128/JCM.05061-11

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title = "Leishmania (Viannia) species identification on clinical samples from cutaneous leishmaniasis patients in Peru: Assessment of a molecular stepwise approach",

abstract = "Wepresent an algorithm based on three PCR assays for Leishmania (Viannia) species identification and assessed its performance using 70 specimens from Peruvian patients. The succession of the assayed targets can be ordered according to species prevalence. Sequential progression through the algorithm reduced the number of samples here studied by approximately 30\% after each step.",

author = "Nicolas Veland and Boggild, \{Andrea K.\} and Cristian Valencia and Valencia, \{Braulio M.\} and Alejandro Llanos-Cuentas and \{Van Der Auwera\}, Gert and Dujardin, \{Jean Claude\} and Jorge Arevalo",

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doi = "10.1128/JCM.05061-11",

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Veland, N, Boggild, AK, Valencia, C, Valencia, BM, Llanos-Cuentas, A, Van Der Auwera, G, Dujardin, JC & Arevalo, J 2012, 'Leishmania (Viannia) species identification on clinical samples from cutaneous leishmaniasis patients in Peru: Assessment of a molecular stepwise approach', Journal of Clinical Microbiology, vol. 50, n.º 2, pp. 495-498. https://doi.org/10.1128/JCM.05061-11

Leishmania (Viannia) species identification on clinical samples from cutaneous leishmaniasis patients in Peru: Assessment of a molecular stepwise approach. / Veland, Nicolas; Boggild, Andrea K.; Valencia, Cristian et al.
En: Journal of Clinical Microbiology, Vol. 50, N.º 2, 02.2012, p. 495-498.

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

TY - JOUR

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T2 - Assessment of a molecular stepwise approach

AU - Veland, Nicolas

AU - Boggild, Andrea K.

AU - Valencia, Cristian

AU - Valencia, Braulio M.

AU - Llanos-Cuentas, Alejandro

AU - Van Der Auwera, Gert

AU - Dujardin, Jean Claude

AU - Arevalo, Jorge

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AB - Wepresent an algorithm based on three PCR assays for Leishmania (Viannia) species identification and assessed its performance using 70 specimens from Peruvian patients. The succession of the assayed targets can be ordered according to species prevalence. Sequential progression through the algorithm reduced the number of samples here studied by approximately 30% after each step.

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Leishmania (Viannia) species identification on clinical samples from cutaneous leishmaniasis patients in Peru: Assessment of a molecular stepwise approach

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